Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous Grass Eragrostis curvula.
Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous Grass Eragrostis curvula.
复制标题
双孢子草无融合生殖与有性基因甲基化模式的差异。
DOI：
10.3390/plants10050946
复制
发表时间：
2021-05-10
期刊：
影响因子：
--
通讯作者：
Echenique V
中科院分区：
文献类型：
--
作者：
Carballo J;Zappacosta D;Marconi G;Gallardo J;Di Marsico M;Gallo CA;Caccamo M;Albertini E;Echenique V
DNA甲基化是一种表观遗传机制，通过该机制甲基基团被添加到胞嘧啶或腺嘌呤。当位于基因/调控序列中时，它可以抑制或去抑制基因，这取决于环境和物种。弯画眉草是一种无融合生殖草，其中兼性基因型增加了由表观遗传机制触发的有性雌蕊的频率。本研究的目的是寻找生殖方式和特定甲基化基因或基因组区域之间的相关性。为此，通过MCSeEd（甲基化环境敏感酶ddRad）研究具有对比生殖模式的植物，其显示在无融合生殖基因型中较高水平的DNA甲基化。此外，增加比例的差异甲基化的位置的监管区域，表明其可能的作用，在基因表达的调控。有趣的是，甲基化途径也被发现是自我调节的，因为参与去甲基化的两个主要基因（ROS 1和ROS 4）在具有不同生殖行为的基因型之间被发现差异甲基化。此外，这项工作使我们能够检测到甲基化调控的几个基因，这些基因以前在无融合生殖和有性基因型之间的比较中发现差异表达，将DNA甲基化与生殖模式的差异联系起来。
DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.
登录
查看更多内容
DOI：
10.1073/pnas.1710683114
发表时间：
2017-08-29
影响因子：
11.1
作者：
Duan, Cheng-Guo;Wang, Xingang;Zhu, Jian-Kang
通讯作者：
Zhu, Jian-Kang
影响因子：
12.3
作者：
Akalin A;Kormaksson M;Li S;Garrett-Bakelman FE;Figueroa ME;Melnick A;Mason CE
通讯作者：
Mason CE
DOI：
10.1534/g3.111.000232
发表时间：
2011-06
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Madzima TF;Mills ES;Gardiner JM;McGinnis KM
通讯作者：
McGinnis KM
DOI：
10.1073/pnas.1402275111
发表时间：
2014-06-10
影响因子：
11.1
作者：
Iwasaki, Mayumi;Paszkowski, Jerzy
通讯作者：
Paszkowski, Jerzy
影响因子：
64.8
作者：
Khanday, Imtiyaz;Skinner, Debra;Sundaresan, Venkatesan
通讯作者：
Sundaresan, Venkatesan