Isolation and activity of Xenorhabdus antimicrobial compounds against the plant pathogens Erwinia amylovora and Phytophthora nicotianae
Isolation and activity of Xenorhabdus antimicrobial compounds against the plant pathogens Erwinia amylovora and Phytophthora nicotianae
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DOI：
10.1111/j.1365-2672.2009.04249.x
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发表时间：
2009-09-01
影响因子：
4
通讯作者：
Taylor, R. A. J.
中科院分区：
文献类型：
--
作者：
Boeszoermenyi, E.;Ersek, T.;Taylor, R. A. J.
目的：利用昆虫病原线虫共生菌产生的广谱抗生素维持土壤中昆虫尸体的单菌条件。本研究评估了抗生素产生的EPB控制植物病原细菌和卵菌的潜力。方法和结果：昆虫病原细菌产生的抗生素有效地对抗火疫病菌欧文氏菌amylovora，包括链霉素抗性菌株，并作为春雷霉素或链霉素在人工气候室实验有效。Xabhabdus budapestensis和X. szentirmaii抗生素抑制烟草疫霉菌的菌落形成和菌丝生长。从X。Budapestensis中，富含精氨酸的级分（双精氨酸）被Amberlite（）XAD 1180吸附，并用甲醇：1nHCl（99：1）洗脱。双角孢菌能使游动孢子失活，并抑制囊孢子的萌发和菌落形成。
Aims:Broad-spectrum antibiotics produced by symbiotic bacteria [entomopathogenic bacterium (EPB)] of entomopathogenic nematodes keep monoxenic conditions in insect cadavers in soil. This study evaluated antibiotics produced by EPB for their potential to control plant pathogenic bacteria and oomycetes.Methods and Results:Entomopathogenic bacterium produce antibiotics effective against the fire blight bacterium Erwinia amylovora, including streptomycin resistant strains, and were as effective in phytotron experiments as kasugamycin or streptomycin. Xenorhabdus budapestensis and X. szentirmaii antibiotics inhibited colony formation and mycelial growth of Phytophthora nicotianae. From X. budapestensis, an arginine-rich fraction (bicornutin) was adsorbed by Amberlite((R)) XAD 1180, and eluted with methanol : 1 n HCI (99 : 1). Bicornutin inactivated zoospores, and inhibited germination and colony formation of cystospores at