Regulation of dorsal in cultured cells by Toll and tube: tube function involves a novel mechanism.
Regulation of dorsal in cultured cells by Toll and tube: tube function involves a novel mechanism.
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Toll 和管对培养细胞背侧的调节：管功能涉及一种新机制。
DOI：
10.1101/gad.9.3.358
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发表时间：
1995
影响因子：
10.5
通讯作者：
Manley,JL
中科院分区：
文献类型：
--
作者：
Norris,JL;Manley,JL
我们之前描述了一种瞬时共转染测定，使我们能够研究培养细胞中果蝇背蛋白 (dl) 的调节。例如，我们发现Toll跨膜受体的过度表达足以引起dl从细胞质重新定位到细胞核。在此，我们提供的数据表明，之前的基因研究表明，管蛋白在 Toll 下游发挥作用，可以以一种新的方式发挥作用，增强 dl 活性。在没有 dl 的情况下，或者当 dl 存在于细胞质中时，在转染细胞的细胞质中也会发现管状结构。但当 dl 定位于细胞核时，管也定位于细胞核。 tube 然后可以通过与 dl 合作或作为 GAL4-tube 融合蛋白来发挥增强报告基因表达的作用。因此，当 dl 移动到细胞核时，管似乎能够充当 dl 的伴侣或护卫者，然后充当转录共激活子。我们还表明，Toll 的胞质内结构域，特别是与白介素-1 受体具有同源性的区域，足以诱导 dl 管核转位。
We described previously a transient cotransfection assay that allows us to study regulation of the Drosophila Dorsal protein (dl) in cultured cells. For example, we showed that over-expression of the Toll transmembrane receptor was sufficient to cause relocalization of dl from the cytoplasm to the nucleus. Here we present data that the tube protein, shown previously by genetic studies to act downstream of Toll, can function in a novel way to enhance dl activity. In the absence of dl, or when dl is cytoplasmic, tube is also found in the cytoplasm of transfected cells. But when dl is localized to the nucleus, so is tube. tube can then function to enhance reporter gene expression, either by cooperation with dl or as a GAL4-tube fusion protein. tube thus appears capable of acting both as a chaperon or escort for dl as it moves to the nucleus, and then as a transcriptional coactivator. We also show that the intracytoplasmic domain of Toll, and specifically the region sharing homology with the interleukin-1 receptor, is sufficient to induce dl-tube nuclear translocation.