Probing the receptor interactions ofan H5 avian influenza virus using a baculovirus expression system and functionalised poly(acrylic acid) ligands
Probing the receptor interactions ofan H5 avian influenza virus using a baculovirus expression system and functionalised poly(acrylic acid) ligands
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DOI：
10.1016/j.bmc.2007.03.085
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发表时间：
2007-06-15
影响因子：
3.5
通讯作者：
Thompson, Catherine I.
中科院分区：
文献类型：
--
作者：
Barclay, Wendy S.;Jones, Ian M.;Thompson, Catherine I.
流感病毒通过与糖蛋白或糖脂上的末端唾液酸（Neu 5Ac）结合而附着于宿主细胞。Neu 5Ac的连接和寡糖中其他碳水化合物的特性都被认为在限制病毒的宿主范围方面发挥作用。在这项研究中，受体特异性的H5禽流感病毒血凝素蛋白，最近感染的人（流感病毒株A/越南/1194/04）已探测使用碳水化合物官能化聚（丙烯酸）聚合物。还开发了一种杆状病毒表达系统，其允许对在预测影响宿主范围转换的位点处工程化的H5 HA突变体的Neu 5Ac结合特异性进行容易且安全的分析。(C)2007爱思唯尔有限公司保留所有权利。
Influenza viruses attach to host cells by binding to terminal sialic acid (Neu5Ac) on glycoproteins or glycolipids. Both the linkage of Neu5Ac and the identity of other carbohydrates within the oligosaccharide are thought to play roles in restricting the host range of the virus. In this study, the receptor specificity of an H5 avian influenza virus haemagglutinin protein that has recently infected man (influenza strain A/Vietnam/1194/04) has been probed using carbohydrate functionalised poly(acrylic acid) polymers. A baculovirus expression system that allows facile and safe analysis of the Neu5Ac binding specificity of mutants of H5 HA engineered at sites that are predicted to effect a switch in host range has also been developed. (C) 2007 Elsevier Ltd. All rights reserved.