Detection of erythromycin and clindamycin resistance genes in group B streptococcal clinical isolates and cervicovaginal-rectal swabs
Detection of erythromycin and clindamycin resistance genes in group B streptococcal clinical isolates and cervicovaginal-rectal swabs
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DOI：
10.1089/mdr.2007.732
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发表时间：
2007-06-01
影响因子：
2.6
通讯作者：
Adelson, Martin E.
中科院分区：
文献类型：
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作者：
Gygax, Scott E.;Schuyler, Jessica A.;Adelson, Martin E.
采用多重聚合酶链式反应检测B组链球菌(GBS)临床分离株及相应宫颈阴道直肠(CVR)拭子DNA中红霉素(EM)和克林霉素(CM)耐药基因ermB、ermTR和mefA/E。我们将这些结果与46株分离物的EM/CM双纸片扩散法进行了比较。鉴于这些基因存在于其他CVR菌群中，并存在于可移动的遗传元件上，因此，PCR检测无法直接从拭子中预测GBS抗性。因此，从纯化的GBS分离株中只能准确检测抗性基因和预测抗性表型。
A multiplex PCR assay was used to detect the erythromycin (EM) and clindamycin (CM) antibiotic resistance genes, ermB, ermTR, and mefA/E, in Group B Streptococcal (GBS) clinical isolates and in DNA extracted from the corresponding cervicovaginal-rectal (CVR) swabs. We compared these results to the standard EM/CM double disk diffusion assay of 46 isolates. Given that these genes are present in other CVR flora and are found on mobile genetic elements, the PCR assay was unable to predict GBS resistance directly from the swabs. Therefore, PCR can only accurately detect resistance genes and predict the resistance phenotype from purified GBS, isolates.