All patients with acute nonlymphocytic leukemia may have a chromosomal defect.
All patients with acute nonlymphocytic leukemia may have a chromosomal defect.
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DOI：
10.1056/nejm198107163050304
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发表时间：
1981-07
期刊：
影响因子：
--
通讯作者：
J. Yunis;C. Bloomfield;K. Ensrud
中科院分区：
文献类型：
--
作者：
J. Yunis;C. Bloomfield;K. Ensrud
以往的骨髓显带染色体研究表明，半数急性非淋巴细胞白血病（ANLL）患者核型正常。为了确定高分辨率染色体分析是否可以检测到额外的异常，我们研究了26例ANLL患者的骨髓，使用甲氨蝶呤细胞同步化以及直接技术。在24名患者中，包括18名未经治疗的患者，获得了足够的有丝分裂。所有表现出克隆性染色体异常，其中涉及平衡易位11例，完全或部分单体10例，三体6例。确定了先前报告的ANLL复发缺陷，包括2例病例的t（15;17），2例病例的-7，3例病例的+8。此外，一个新的具体异常涉及带11 q23指出，在1例急性单核细胞白血病和2例髓单核细胞白血病。我们的研究结果表明，大多数（如果不是全部）ANLL患者有染色体变化，我们的新技术可以更精确地识别具有特征性临床和血液学特征的ANLL亚型。
Previous studies of banded marrow chromosomes suggest that half the patients with acute nonlymphocytic leukemia (ANLL) have normal karyotypes. To determine whether high-resolution chromosome analysis could detect additional abnormalities, we studied marrow from 26 patients with ANLL, using methotrexate cell synchronization as well as a direct technique. In 24 patients, including 18 who were untreated, adequate mitoses were obtained. All demonstrated clonal chromosomal abnormalities, which involved a balanced translocation in 11 cases, a complete or partial monosomy in 10, and a trisomy in six. Previously reported recurring defects in ANLL were identified, including t(15;17) in two cases, -7 in two cases, and +8 in three cases. In addition, a new specific abnormality involving band 11q23 was noted in one patient with acute monocytic leukemia and in two with myelomonocytic leukemia. Our results suggest that most, if not all, patients with ANLL have chromosomal changes, and that our new technique may allow more precise identification of subtypes of ANLL with characteristic clinical and hematologic features.