5 `-thiolated oligonucleotides on (3-mercaptopropyl)trimethoxysilane-mica: Surface topography and coverage
5 `-thiolated oligonucleotides on (3-mercaptopropyl)trimethoxysilane-mica: Surface topography and coverage
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DOI：
10.1021/la027039x
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发表时间：
2003-06
期刊：
影响因子：
3.9
通讯作者：
M. Cheung;D. Trau;K. Yeung;M. Carles;N. Sucher
中科院分区：
文献类型：
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作者：
M. Cheung;D. Trau;K. Yeung;M. Carles;N. Sucher
DNA（脱氧核糖核酸）微阵列被用作高通量方法获取遗传信息的工具。利用原子力显微镜、x射线光电子能谱（XPS）、飞行时间二次离子质谱和共聚焦扫描荧光显微镜，研究了（3-巯基丙基）三甲氧基硅烷（MPTS）涂层云母的表面性质和形貌对5′-硫代寡核苷酸固定的影响。接枝云母表面的MPTS浓度变化超过3个数量级（5.38 ~ 5.38 M）。在低浓度（5.38 mM）下，浓缩的MPTS呈现“岛状”结构，平均表面积为100 nm2。随着MPTS浓度的增加，MPTS岛的大小增加到2400 nm2，表明MPTS单层的形成。XPS分析表明，硫元素浓度相应增加。在高MPTS浓度（107.6 mM和5.38 M）下，岛状结构消失。
DNA (deoxyribonucleic acid) microarrays are used as a tool in high-throughput methods to obtain genetic information. Using atomic force microscopy, X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry, and confocal scanning fluorescence microscopy, we investigated how the surface properties and topography of (3-mercaptopropyl)trimethoxysilane (MPTS)-coated mica influence the immobilization of 5‘-thiolated oligonucleotides. Mica surfaces were grafted with concentrations of MPTS varied over 3 orders of magnitude (5.38 mM to 5.38 M). At low concentration (5.38 mM), condensed MPTS appeared to form “island-like” structures with an average surface area of 100 nm2. The MPTS islands grew in size to 2400 nm2 as the concentration of MPTS was increased, suggesting the formation of an MPTS monolayer. XPS analysis indicated a corresponding increase in the elemental concentration of sulfur. At high MPTS concentrations (107.6 mM and 5.38 M), the island structures disappeared, suggesting...