Hydrogen sulfide improves drought resistance in Arabidopsis thaliana
Hydrogen sulfide improves drought resistance in Arabidopsis thaliana
复制标题
硫化氢提高拟南芥的抗旱性
DOI：
10.1016/j.bbrc.2011.09.090
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发表时间：
2011-10-28
影响因子：
3.1
通讯作者：
Pei, Yanxi
中科院分区：
文献类型：
--
作者：
Jin, Zhuping;Shen, Jiejie;Pei, Yanxi
硫化氢（H（2）S）在人类和动物的生理中起着至关重要的作用。它的普遍性和多功能特性最近引起了植物生理学家和生物化学家的注意。L-半胱氨酸脱氢酶和D-半胱氨酸脱氢酶是两种半胱氨酸脱氢酶，它们主要负责半胱氨酸的降解以产生H（2）S。本研究探讨了这些基因的表达调控及其与拟南芥耐旱性的关系。首先，研究了CDes在拟南芥中的表达模式。CDes的表达水平随着年龄的增长而逐渐增加。CDes在茎和茎生叶中的表达量显著高于根、莲座叶和花。第二，评价了硫化氢对干旱的保护作用。CDes的表达模式与干旱诱导的干旱相关基因相似，H（2）S熏蒸进一步刺激了干旱相关基因的表达。干旱也显著诱导增加H（2）S的生产，这一过程被逆转复水。此外，NaHS（一种H（2）S供体）处理后的幼苗表现出较高的存活率，并且气孔孔径比对照显著减小。这些结果为H（2）S作为气体传递物质提高拟南芥的抗旱性提供了证据。(C)2011 Elsevier Inc. All rights reserved.
Hydrogen sulfide (H(2)S) plays a crucial role in human and animal physiology. Its ubiquity and versatile properties have recently caught the attention of plant physiologists and biochemists. Two cysteine desulfhydrases (CDes), L-cysteine desulfhydrase and D-cysteine desulfhydrase, were identified as being mainly responsible for the degradation of cysteine in order to generate H(2)S. This study investigated the expression regulation of these genes and their relationship to drought tolerance in Arabidopsis. First, the expression pattern of CDes in Arabidopsis was investigated. The expression levels of CDes gradually increased in an age-dependent manner. The expression of CDes was significantly higher in stems and cauline leaves than in roots, rosette leaves and flowers. Second, the protective effect of H(2)S against drought was evaluated. The expression pattern of CDes was similar to the drought associated genes induced by dehydration, and H(2)S fumigation was found to stimulate further the expression of drought associated genes. Drought also significantly induced increased H(2)S production, a process that was reversed by rewatering. In addition, seedlings after treatment with NaHS (a H(2)S donor) showed a higher survival rate and displayed a significant reduction in the size of the stomatal aperture compared to the control. These findings provide evidence that H(2)S, as a gasotransmitter, improves drought resistance in Arabidopsis. (C) 2011 Elsevier Inc. All rights reserved.