Mass spectrometry for monitoring respiratory and anesthetic gas waveforms in rats.
Mass spectrometry for monitoring respiratory and anesthetic gas waveforms in rats.
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用于监测大鼠呼吸和麻醉气体波形的质谱法。
DOI：
10.1152/jappl.1988.65.2.955
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Derr,JA
中科院分区：
文献类型：
--
作者：
Larach,DR;Schuler,HG;Skeehan,TM;Derr,JA
提出了一种实时监测大鼠和其他小动物气道气体浓度波形的方法。从气管导管中抽取气体，通过质谱仪进行分析，并同时显示为CO2、氟烷和其他呼吸气体和麻醉剂的浓度与时间波形。利用呼吸模拟装置，将质谱法分析呼气末CO2的准确性与实际气体成分和红外分光光度法进行了比较。还检查了各种呼吸机速率和吸气呼气比对采样准确性的影响。该技术在接受机械通气的雄性Sprague-Dawley大鼠中得到了验证。动脉PCO 2（PaCO 2）和潮气末PCO 2（PETCO 2）之间的差异无显著性差异，PETCO 2和PaCO 2之间的相关性很强（r = 0.97，P <0.0001）。持续气体采样长达5分钟不影响PaCO 2、PETCO 2或气道压力。通过使用这种新方法测量约6.5月龄大鼠的潮气末氟烷浓度，阻止响应夹尾的反射运动的氟烷的最小肺泡浓度为0.97 +/- 0.04%大气压（n = 14）。这种质谱技术可用于小型实验室动物，如体重仅为250 g的大鼠。气体监测没有扭曲PETCO 2或PaCO 2。在本研究规定的条件下，可以实现麻醉和呼吸气体的阶段性呼吸浓度的准确和同时测量。
A method is presented for real-time monitoring of airway gas concentration waveforms in rats and other small animals. Gas is drawn from the tracheal tube, analyzed by a mass spectrometer, and presented as concentration vs. time waveforms simultaneously for CO2, halothane, and other respiratory gases and anesthetics. By use of a respiratory simulation device, the accuracy of mass spectrometric end-tidal CO2 analysis was compared with both the actual gas composition and infrared spectrophotometry. The effects of various ventilator rates and inspiration-to-expiration ratios on sampling accuracy were also examined. The technique was validated in male Sprague-Dawley rats being ventilated mechanically. The difference between the arterial PCO2 (PaCO2) and the end-tidal PCO2 (PETCO2) was not significantly different from zero, and the correlation between PETCO2 and PaCO2 was strong (r = 0.97, P less than 0.0001). Continuous gas sampling for periods up to 5 min did not affect PaCO2, PETCO2, or airway pressures. By use of this new method for measuring end-tidal halothane concentrations in rats approximately 6.5 mo of age, the minimum alveolar concentration of halothane that prevented reflex movement in response to tail clamping was 0.97 +/- 0.04% atmospheric (n = 14). This mass spectrometric technique can be used in small laboratory animals, such as rats, weighing as little as 250 g. Gas monitoring did not distort either PETCO2 or PaCO2. Under the defined conditions of this study, accurate and simultaneous measurements of phasic respiratory concentrations of anesthetic and respiratory gases can be achieved.