Cell-Membrane Permeable Redox Phospholipid Polymers Induce Apoptosis in MDA-MB-231 Human Breast Cancer Cells
Cell-Membrane Permeable Redox Phospholipid Polymers Induce Apoptosis in MDA-MB-231 Human Breast Cancer Cells
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DOI：
10.1021/acs.biomac.9b01184
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发表时间：
2019-12-01
影响因子：
6.2
通讯作者：
Nakanishi, Shuji
中科院分区：
文献类型：
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作者：
Kaneko, Masahiro;Ishikawa, Masahito;Nakanishi, Shuji
诱导氧化应激是导致癌细胞凋亡的有效途径。由于氧化应激通常是由细胞内氧化还原失衡引起的，因此改变细胞内氧化还原是抑制癌细胞生长的一种有前途的策略。在这里，我们试图通过加入细胞膜可渗透的氧化还原磷脂聚合物，可以改变细胞内的氧化还原，诱导MDA-MB-231人乳腺癌细胞凋亡。我们发现，在氧化还原聚合物的氧化形式的存在下，在MDA-MB-231细胞中诱导凋亡和氧化磷酸化失活。值得注意的是，在不能拦截代谢电子的还原形式的氧化还原聚合物的存在下没有观察到这种现象。这些结果表明，介导细胞外电子转移（EET）的氧化还原聚合物产生氧化应激，导致癌细胞凋亡。
Induction of oxidative stress is an effective approach to causing apoptotic death of cancer cells. Since oxidative stress is generally caused by an intracellular redox imbalance, altering the intracellular redox is a promising strategy toward the growth suppression of cancer cells. Here, we attempted to induce apoptosis in MDA-MB-231 human breast cancer cells by adding a cell-membrane permeable redox phospholipid polymer that can alter the intracellular redox. We found that apoptosis and the deactivation of oxidative phosphorylation were induced in the MDA-MB-231 cells in the presence of the oxidized form of the redox polymer. Remarkably, such phenomena were not observed in the presence of the reduced form of the redox polymer that cannot intercept metabolic electrons. These results indicate that the redox polymer that mediates extracellular electron transfer (EET) generates oxidative stress, leading to the apoptosis of the cancer cells.