A method for PCR-based identification of fish eggs attached to vegetation in Lake Biwa, Japan
A method for PCR-based identification of fish eggs attached to vegetation in Lake Biwa, Japan
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基于 PCR 的日本琵琶湖植被附着鱼卵鉴定方法
DOI：
10.1007/s12686-016-0536-9
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发表时间：
2016
影响因子：
1.1
通讯作者：
Mabuchi K
中科院分区：
文献类型：
--
作者：
Hiromi Sano;Kenji F Tanaka;Atsushi Nambu;Mabuchi K
设计了四个属的单管基因分型引物，分别为鲤属、鲤属、金鱼属和石龙属。琵琶湖属于这四个属的六种物种(一种是日本本土鲤鱼)大多濒临灭绝，六种中的两种是重要的渔业资源。然而，它们的生殖生态的许多方面都不清楚，因为这些物种在一年中的同一时间在相似的底物上产卵，看起来相似。这里开发的分子方法使高效和经济的属水平鉴定成为可能，为制定有效的保护这些物种的策略提供了突破。
Primers were developed for single-tube genotyping of four genera,Cyprinus,Carassius,Gnathopogon, andIschikauia. Most of the six species in Lake Biwa belonging to these four genera (one is a native Japanese common carp) are endangered, and two of the six are important fisheries resources. However, many aspects of their reproductive ecology are unclear, because these species spawn similar looking eggs on similar substrates during the same time of year. The molecular method developed here enabled efficient and cost-effective genus-level identification of such eggs, providing a breakthrough for developing effective conservation strategies for these species.