Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent
Kinetics of catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent
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DOI:
10.1016/j.saa.2011.05.013
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发表时间:
2011-09-01
影响因子:
4.4
通讯作者:
Ebeid, El-Zeiny M.
中科院分区:
文献类型:
--
作者:
Al-Kady, Ahmed S.;Ahmed, El-Sadat I.;Ebeid, El-Zeiny M.
The kinetics of chemical hydrolysis including neutral, acid- and base-catalyzed hydrolysis of 4-methylumbelliferyl caprylate (MUCAP) salmonella reagent were studied at different temperatures. The rate constants and activation parameters were determined by following the build-up of fluorescence peak of the hydrolysis product 4-methylumbelliferone (4-MU). The time scale of esterase enzyme hydrolysis caused by salmonella was compared with chemical hydrolysis as a background process. (C) 2011 Elsevier B.V. All rights reserved.