A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids
A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids
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DOI：
10.1016/s1097-2765(02)00474-4
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发表时间：
2002-03-01
期刊：
影响因子：
16
通讯作者：
Ahlquist, P
中科院分区：
文献类型：
--
作者：
Schwartz, M;Chen, JB;Ahlquist, P
我们发现，雀麦花叶病毒(BMV)RNA复制蛋白1a、2a聚合酶和一个顺式作用的复制信号概括了传统逆转录病毒和泡沫病毒核心中Gag、Pol和RNA包装信号的功能。在RNA复制之前，La形成球状发芽进入内质网膜，将病毒正链RNA模板隔离在抗核酸酶、对洗涤剂敏感的状态。当表达时，2a聚合酶共定位在这些球体中，这些球体成为病毒RNA合成的位置，并保留负链模板用于正链RNA合成。这些结果解释了许多正链RNA病毒复制的许多特征，并揭示了这些病毒、反转录病毒和dsRNA病毒在复制方面有基本相似之处，可能具有共同的进化起源。
We show that brome mosaic virus (BMV) RNA replication protein 1a, 2a polymerase, and a cis-acting replication signal recapitulate the functions of Gag, Pol, and RNA packaging signals in conventional retrovirus and foamy virus cores. Prior to RNA replication, la forms spherules budding into the endoplasmic reticulum membrane, sequestering viral positive-strand RNA templates in a nuclease-resistant, detergent-susceptible state. When expressed, 2a polymerase colocalizes in these spherules, which become the sites of viral RNA synthesis and retain negative-strand templates for positive-strand RNA synthesis. These results explain many features of replication by numerous positive strand RNA viruses and reveal that these viruses, reverse transcribing viruses, and dsRNA viruses share fundamental similarities in replication and may have common evolutionary origins.