Physiological subgroups of nonpyramidal cells with specific morphological characteristics in layer II/III of rat frontal cortex
Physiological subgroups of nonpyramidal cells with specific morphological characteristics in layer II/III of rat frontal cortex
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DOI：
10.1523/jneurosci.15-04-02638.1995
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发表时间：
1995-04
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影响因子：
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通讯作者：
Y. Kawaguchi
中科院分区：
文献类型：
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作者：
Y. Kawaguchi
采用全细胞记录和细胞内生物细胞素染色的方法，研究了幼年大鼠额叶皮层第II/III层非锥体细胞的生理形态学特征。第II/III层非锥体细胞在去极化电流脉冲下的放电模式及其树突和轴突的树突分布模式可分为4个亚群。(1)快速尖峰（FS）细胞（占总样本的35%）表现出短时间动作电位的非适应性重复放电的突然发作。FS细胞具有局部和水平轴突，但未进入第一层。这种类型的FS细胞对小白蛋白有免疫反应，包括一些篮状细胞。三个吊灯细胞被鉴定为FS细胞，尽管一个吊灯细胞未被鉴定为FS细胞。(2)晚尖峰细胞（11%）在阈值附近表现出缓慢发展的斜坡去极化。LS细胞为神经胶质样细胞。(3)低阈值尖峰（LTS）细胞（5%）在超极化刺激下具有显著的低阈值尖峰。LTS细胞的主轴突上升，侧枝进入第1层。(4)其余的细胞[规则尖峰非锥体（RSNP）细胞]（49%）不能划分为上述三个亚群。在一些RSNP细胞中，来自超极化的去极化脉冲诱导了小振幅的快速去极化缺口。RSNP细胞轴突场垂直伸长，从第1层延伸到第5层，有时延伸到第6层。该亚群包括双花束细胞和双极细胞。具有不同放电模式的每个亚群可能对新皮层层流和柱状回路有不同的贡献。
Physiological and morphological properties of nonpyramidal cells in layer II/III of frontal cortex of young rats were studied in vitro by whole-cell recording and intracellular staining with biocytin. Layer II/III nonpyramidal cells could be divided into four subgroups by their firing patterns in response to depolarizing current pulses and their patterns of dendritic and axonal arborizations. (1) Fast-spiking (FS) cells (35% of the total sample) showed an abrupt episode of nonadapting repetitive discharges of shorter-duration action potentials. FS cells had local and horizontal axonal arbors which did not enter layer I. This type of FS cell was immunoreactive for parvalbumin and included some basket cells. Three chandelier cells were identified as FS cells, although one chandelier cell was not identified as an FS cell. (2) Late- spiking (LS) cells (11%) exhibited slowly developing ramp depolarizations near threshold. LS cells were neurogliaform cells. (3) Low-threshold spike (LTS) cells (5%) had prominent low-threshold spikes when stimulated from hyperpolarizations. The main axons of LTS cells ascended, and the collaterals entered into layer I. (4) The remaining cells [regular-spiking nonpyramidal (RSNP) cells] (49%) could not be categorized into the above three subgroups. In some RSNP cells depolarizing pulses from hyperpolarizations induced fast depolarizing notches with small amplitude. RSNP cells had vertically elongated axonal fields, extending from layer I to V, sometimes to layer VI. This subgroup included double bouquet cells and bipolar cells. Each subgroup with a different firing mode may differentially contribute to neocortical laminar and columnar circuitry.