Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.
Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.
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DOI：
10.1364/boe.3.002752
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Fischer MC
中科院分区：
文献类型：
--
作者：
Wilson JW;Degan S;Warren WS;Fischer MC
标准的组织病理学技术（包括石蜡包埋）与厚组织多光子成像不兼容，并且这些标本上的标准清除技术会破坏一些分子信息。我们展示了根据标准组织病理学技术制备的标本中的多光子成像。这使得档案组织库的未标记的三维组织学，这是评估预后指标的重要价值。
Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.