Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation
Cyclosporine facilitates chimeric and inhibits nonchimeric tolerance after posttransplant total lymphoid irradiation
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DOI：
10.1097/00007890-200002270-00029
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发表时间：
2000-02-27
期刊：
影响因子：
6.2
通讯作者：
Strober, S
中科院分区：
文献类型：
--
作者：
Lan, FS;Hayamizu, K;Strober, S
背景资料。以往的研究表明，移植后接受全淋巴照射、抗胸腺细胞球蛋白和单次输注ACI外周血或骨髓细胞的Lewis大鼠对ACI同种异体心脏移植物产生耐受。为了确定环孢素对这些耐受诱导方案的影响，接受ACI血液或骨髓输注的Lewis宿主组在细胞输注后立即给予每日60天的环孢素疗程。在接受ACI输血的组中，环孢素治疗与均匀的移植物排斥反应有关，在接受ACI骨髓输注的组中，环孢素治疗与一致的移植物接受性相关，对宿主血液中供者类型T和B细胞嵌合体的研究表明，环孢素促进了接受ACI骨髓细胞的宿主的嵌合体，并通过在第30天可检测到的嵌合体来预测300天观察期内的稳定嵌合体。给予ACI血细胞的宿主均未发生嵌合体。环孢素在诱导混合嵌合体的耐受性方案中促进了长期的移植物接受，但在不诱导嵌合体的耐受性方案中阻止了移植物的长期接受。
Background. previous studies showed that Lewis rats given posttransplant total lymphoid irradiation, antithymocyte globulin, and a single infusion of ACI peripheral blood or bone marrow cells develop tolerance to ACI heart allografts.Methods. To determine the effects of cyclosporine on these tolerance induction protocols, groups of Lewis hosts, given either ACI blood or marrow infusions, were given a 60-day course of daily cyclosporine immediately after the cell infusion.Results. Cyclosporine treatment was associated with uniform graft rejection in the groups given an ACI blood transfusion, and was associated with uniform graft acceptance in the groups given an ACI bone marrow infusion, Studies of donor-type T and B cell chimerism in the host blood showed that cyclosporine facilitated chimerism in the hosts given ACI bone marrow cells, and stable chimerism over a 300-day observation period was predicted by detectable chimerism by day 30. None of the hosts given ACI blood cells developed chimerism.Conclusion. Cyclosporine facilitated long-term graft acceptance in a tolerization protocol that induced mixed chimerism, but prevented long-term graft acceptance in a tolerization protocol that did not induce chimerism.