Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis
Role of the ZWILLE gene in the regulation of central shoot meristem cell fate during Arabidopsis embryogenesis
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DOI：
10.1093/emboj/17.6.1799
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发表时间：
1998-03-16
期刊：
影响因子：
11.4
通讯作者：
Laux, T
中科院分区：
文献类型：
--
作者：
Moussian, B;Schoof, H;Laux, T
高等植物胚后发育的特征是通过自我延续的干细胞系统（茎分生组织）重复器官形成。器官起始于茎分生组织的外围，而中心区则容纳干细胞。在这里，我们通过遗传和分子分析表明，ZWILLE（ZLL）基因是专门需要建立中央外周组织的胚尖，这一步是至关重要的拍摄分生组织自我永存。ZLL突变体在早期胚胎中正确启动了茎分生组织特异基因SHOOT MERISTEMLESS的表达，但在后期胚胎阶段不能调控其空间表达模式，并启动了替代干细胞的分化结构。它编码一种新的蛋白质，相关序列在多细胞植物和动物中高度保守，但在细菌和酵母中不存在。我们建议，ZLL继电器的位置信息，需要保持在未分化状态的干细胞的发展芽分生组织在从胚胎发育到重复胚后器官形成的过渡。
Postembryonic development in higher plants is marked by repetitive organ formation via a self-perpetuating stem cell system, the shoot meristem, Organs are initiated at the shoot meristem periphery, while a central zone harbors the stem cells. Here we show by genetic and molecular analyses that the ZWILLE (ZLL) gene is specifically required to establish the central-peripheral organization of the embryo apex and that this step is critical for shoot meristem self-perpetuation. zll mutants correctly initiate expression of the shoot meristem-specific gene SHOOT MERISTEMLESS in early embryos, but fail to regulate its spatial expression pattern at later embryo stages and initiate differentiated structures in place of stem cells, We isolated the ZLL gene by map-based cloning. It encodes a novel protein, and related sequences are highly conserved in multicellular plants and animals but are absent from bacteria and yeast. We propose that ZLL relays positional information required to maintain stem cells of the developing shoot meristem in an undifferentiated state during the transition from embryonic development to repetitive postembryonic organ formation.