Photolytic protein aggregates: versatile materials for controlled release of active proteins
Photolytic protein aggregates: versatile materials for controlled release of active proteins
复制标题
光解蛋白质聚集体：用于控制释放活性蛋白质的多功能材料
DOI：
10.1002/adhm.201500957
复制
发表时间：
2016
影响因子：
10
通讯作者：
T. Nagamune
中科院分区：
文献类型：
--
作者：
A. Ishiwatari;S. Yamaguchi;S. Takamori;S. Yamahira;K. Minamihata;T. Nagamune
光解蛋白聚集体是光诱导释放活性蛋白的一种简便、通用的平台。生物素修饰的蛋白质通过光可切割的连接物与链霉亲和素和生物素化的功能分子通过简单的混合迅速形成聚集体。光照射从聚集体中高产量释放活性蛋白，并且成功地证明了光诱导药物修饰转铁蛋白进入活细胞的摄取。
Photolytic protein aggregates are developed as a facile and versatile platform for light-induced release of active proteins. The proteins modified with biotin through a photo-cleavable linker rapidly form aggregates with streptavidin and biotinylated functional molecules simply by mixing. Light irradiation releases active proteins from the aggregates in high yields, and light-induced uptake of drug-modified transferrin into living cells is successfully demonstrated.