COUGH PRODUCTION, LEUKOCYTOSIS AND SEROLOGY OF RATS INFECTED INTRABRONCHIALLY WITH BORDETELLA-PERTUSSIS
COUGH PRODUCTION, LEUKOCYTOSIS AND SEROLOGY OF RATS INFECTED INTRABRONCHIALLY WITH BORDETELLA-PERTUSSIS
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DOI：
10.1099/00222615-40-3-205
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发表时间：
1994-03-01
影响因子：
3
通讯作者：
WARDLAW, AC
中科院分区：
文献类型：
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作者：
HALL, E;PARTON, R;WARDLAW, AC
成年Sprague-Dawley大鼠支气管内感染包在琼脂糖珠（BP珠）中的贝氏博德特氏菌百日咳菌株18-323，出现阵发性咳嗽和白细胞增多，两者均在第10天左右达到峰值。当动物暴露于乙醚2分钟后，交付的珠，有一个后续咳嗽发作的数量增加。在珠子中包含角叉菜胶也增强了咳嗽。对照组大鼠，给予无菌珠或不治疗，仅显示出低水平的咳嗽或不咳嗽，这取决于它们的来源。用热灭活B通过相同途径激发大鼠。珠粒中的百日咳或悬浮液中的活生物体（无珠粒）未显示咳嗽诱导或白细胞增多。然而，鼻内递送B.百日咳混悬液引起中度咳嗽和白细胞增多。血清IgG对B的反应。百日咳抗原在用BP-珠感染的大鼠中最大，并且检测到针对百日咳毒素和丝状血凝素的抗体。由于大鼠是唯一方便获得的实验动物物种，其中B。百日咳是间歇性的。与人类一样，百日咳是一种典型的阵发性咳嗽，因此，对于确定百日咳的发病机制和免疫机制，值得进一步研究。
Adult Sprague-Dawley rats infected intrabronchially with Bordetella pei pertussis strain 18-323 encased in agarose beads (BP-beads), developed a paroxysmal cough and leucocytosis, both of which peaked at around day 10. When animals were exposed to ether for 2 min after delivery of the beads, there was an enhancement of the number of subsequent coughing episodes. Inclusion of carrageenan in the beads also enhanced coughing. Control rats, given sterile beads or left untreated, showed only a low level of coughing or no coughing, depending upon their source. Rats challenged by the same route with heat-killed B. pertussis in beads, or with live organisms in suspension (without beads) showed no cough induction or leucocytosis. However, intranasal delivery of B. pertussis suspension gave rise to a moderate amount of coughing and leucocytosis. Serum IgG responses to B. pertussis antigens were greatest in rats infected with BP-beads and antibodies against both pertussis toxin and filamentous haemagglutinin were detected. Since the rat is the only conveniently accessible laboratory animal species in which B. pertussis induces an intermittent. paroxysmal cough, as in man, it merits further study for determining the mechanisms of pathogenesis and immunity in pertussis.