Progesterone enhances macrophage colony-stimulating factor production in human endometrial stromal cells in vitro.
Progesterone enhances macrophage colony-stimulating factor production in human endometrial stromal cells in vitro.
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DOI：
10.1210/endo.135.5.7956912
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发表时间：
1994-11
期刊：
影响因子：
4.8
通讯作者：
H. Hatayama;H. Kanzaki;M. Iwai;M. Kariya;M. Fujimoto;Toshihiko Higuchi;K. Kojima;Hiroki Nakayama;Takahide Mori;Jun Fujita
中科院分区：
文献类型：
--
作者：
H. Hatayama;H. Kanzaki;M. Iwai;M. Kariya;M. Fujimoto;Toshihiko Higuchi;K. Kojima;Hiroki Nakayama;Takahide Mori;Jun Fujita
越来越多的证据表明，巨噬细胞集落刺激因子（M-CSF）产生于子宫内膜，并在生殖过程中发挥重要作用。本研究采用体外蜕膜化模型和人子宫内膜，通过北方印迹和原位杂交技术，研究了人子宫内膜间质细胞（ESC）中M-CSF信使RNA（mRNA）的表达。用酶联免疫吸附法检测ESC培养液中M-CSF的分泌量。在孕酮（P）或雌激素存在下培养ESC。在用P培养9天后，当通过PRL的产生证实体外蜕膜化时，M-CSF mRNA和蛋白水平分别比不含P的培养物高3.1 +/- 0.5和3.2 +/- 0.8倍（平均值+/- SEM）（P < 0.01）。P诱导的增加具有剂量依赖性。另一方面，雌激素不增加M-CSF mRNA的表达。M-CSFmRNA在表达PRL mRNA的早孕蜕膜中的表达高于蜕膜。通过原位杂交，ESC以及上皮细胞显示表达M-CSF在体外和体内。这些结果表明，人胚胎干细胞（蜕膜细胞）表达M-CSF mRNA，并建议他们分泌的M-CSF在P-依赖的方式在蜕膜化过程中。
Increasing evidence suggests that macrophage colony-stimulating factor (M-CSF) is produced in the uterine endometrium and that it plays an important role in the reproductive process. In the present study, using an in vitro decidualization model and human endometrium, we investigated M-CSF messenger RNA (mRNA) expression in human endometrial stromal cells (ESC) by Northern blotting and in situ hybridization. The secreted M-CSF in the culture medium of ESC was measured by enzyme-linked immunosorbent assay. ESC were cultured in the presence of progesterone (P) or estrogen. After a 9-day culture with P, when in vitro decidualization was confirmed by the production of PRL, M-CSF mRNA and protein levels were 3.1 +/- 0.5- and 3.2 +/- 0.8-fold (mean +/- SEM) higher, respectively, than those in cultures without P (P < 0.01). The P-induced increase was dose dependent. On the other hand, estrogen did not increase M-CSF mRNA expression. M-CSF mRNA expression in the first trimester deciduae that expressed PRL mRNA was higher than that in the endometria. By in situ hybridization, ESC as well as epithelial cells were shown to express M-CSF both in vitro and in vivo. These findings indicate that human ESC (decidua cells) express M-CSF mRNA and suggest that they secrete M-CSF in a P-dependent manner during the process of decidualization.