Changing patterns of gene expression in the pulmonary trunk-banded rat heart.
Changing patterns of gene expression in the pulmonary trunk-banded rat heart.
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肺干带状大鼠心脏基因表达模式的变化。
DOI：
10.1006/jmcc.1998.0753
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发表时间：
1998
影响因子：
5
通讯作者：
A. Moorman
中科院分区：
文献类型：
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作者：
Ronald H. LekanneDeprez;M. Hoff;P. A. J. Boer;J. Ruijter;Adrie Maas;R. Chamuleau;W. Lamers;A. Moorman
通过捆扎肺干（PT）建立了大鼠压力过载模型，以研究从代偿性右心室（RV）肥大到右心衰竭过渡期间左心室和右心室基因表达的变化。大鼠右心衰竭的特征是肝硬化、胸水和腹水。发现收缩的直径可以确定心力衰竭发展的时间过程。只有 RV 游离壁和右心房重量增加，补偿性 RV 和故障 RV 之间没有差异。循环 ANP 的增加揭示了心肌的肥大反应，而循环氨水平的增加则区分代偿性肥大和衰竭。作为应激、纤维化和 Ca2+ 处理的参数，通过 Northern 印迹和原位杂交分析，研究了编码心房钠尿肽 (ANP)、胶原蛋白 IIIα1 和肌浆内质网状钙 ATP 酶 2 (SERCA2)、受磷蛋白 (PLB) 和钙螯合蛋白 (CSQ) 的 mRNA 模式和水平的变化。肺干束带导致左心室和右心室中 ANP mRNA 的诱导、胶原蛋白 III α1 mRNA 的适度增加以及 SERCA2 和 PLB mRNA 水平的降低，但变化在 RV 腔周围的心肌中最为明显。血液氨水平升高是检测右心衰竭发展的一个有希望的预后标志物。
A pressure-overload model in the rat by banding the pulmonary trunk (PT) was developed to investigate alterations in gene expression in left- and right-ventricular compartments during the transition from compensated right-ventricular (RV) hypertrophy to right heart failure. Right heart failure in rat is characterized by liver cirrhosis, hydrothorax and ascites. The diameter of constriction was found to determine the time course of heart failure development. Only the RV free wall and the right atrium increased in weight, without a difference between compensated and failing RV. An increase in circulating ANP revealed a hypertrophic response of the myocardium, while increased circulating ammonia levels discriminated between compensated hypertrophy and failure. As parameters for stress, fibrosis and Ca2+-handling, changes in the pattern and level of the mRNAs encoding atrial natriuretic peptide (ANP), collagenIIIalpha1, and sarcoplasmic endoplasmic reticular calcium ATPase 2 (SERCA2), phospholamban (PLB) and calsequestrin (CSQ) were studied by Northern blot and in situ hybridization analyses. Pulmonary trunk banding resulted in an induction of ANP mRNA, a moderate increase in collagenIII alpha1 mRNA and a decrease in SERCA2 and PLB mRNA levels in both the left and right ventricles, but changes were most pronounced in the myocardium surrounding the RV cavity. Increased ammonia blood levels are a promising prognostic marker to detect the development of right heart failure.
影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs