Absorption and Excretion of 14C-Perfluorooctanoic Acid (PFOA) in Angus Cattle (Bos taurus)
Absorption and Excretion of 14C-Perfluorooctanoic Acid (PFOA) in Angus Cattle (Bos taurus)
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DOI：
10.1021/jf2042505
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发表时间：
2012-02-01
影响因子：
6.1
通讯作者：
Johnston, John J.
中科院分区：
文献类型：
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作者：
Lupton, Sara J.;Huwe, Janice K.;Johnston, John J.
全氟烷基物质（PFASs），如全氟辛酸（PFOA），是一种环境持久性工业化学品，通常存在于生物固体中。将这些生物固体应用于牧场引起了对全氟辛酸在食用动物可食用组织中积累的担忧。由于无法获得关于PFOA在牛中的吸收、分布、代谢和排泄（ADME）的数据，因此进行了一项研究，以确定4头Lowline安格斯阉牛单次口服暴露（1 mg/kg体重的C-14-PFOA）后的药代动力学参数。通过液体闪烁计数（LSC）或LSC燃烧分析（通过液相色谱-串联质谱法（LC-MS/MS）确认），对血液、尿液和粪便中的放射性碳进行定量（持续28天），并在屠宰时（28天）对组织中的放射性碳进行定量。在给药后9天内，C-14-PFOA被完全吸收并经尿液排泄（100.7 +/- 3.3%回收率）。血浆消除半衰期为19.2 +/- 3.3 h。在可食用组织中未检测到C-14-PFOA衍生的放射性。虽然PFOA被迅速吸收，但它也被阉牛迅速排出，并且不会持续存在于可食用组织中，这表明接触急性剂量PFOA的牛肉不太可能成为人类接触的主要来源。
Perfluoroalkyl substances (PFASs), such as perfluorooctanoic acid (PFOA), are environmentally persistent industrial chemicals often found in biosolids. Application of these biosolids to pastures raises concern about the accumulation of PFOA in the edible tissues of food animals. Because data on the absorption, distribution, metabolism, and excretion (ADME) of PFOA in cattle were unavailable, a study was conducted to determine pharmacokinetic parameters following a single oral exposure (1 mg/kg body weight of C-14-PFOA) in four Lowline Angus steers. Radiocarbon was quantified in blood, urine, and feces for 28 days and in tissues at the time of slaughter (28 days) by liquid scintillation counting (LSC) or by combustion analysis with LSC with confirmation by liquid chromatography-tandem mass spectrometry (LC-MS/MS). C-14-PFOA was completely absorbed and excreted (100.7 +/- 3.3% recovery) in the urine within 9 days of dosing. The plasma elimination half-life was 19.2 +/- 3.3 h. No C-14-PFOA-derived radioactivity was detected in edible tissues. Although PFOA was rapidly absorbed, it was also rapidly excreted by steers and did not persist in edible tissues, suggesting meat from cattle exposed to an acute dose of PFOA is unlikely to be a major source of exposure to humans.