The inflammatory microenvironment in colorectal neoplasia.
The inflammatory microenvironment in colorectal neoplasia.
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DOI：
10.1371/journal.pone.0015366
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发表时间：
2011-01-07
期刊：
影响因子：
3.7
通讯作者：
El-Omar EM
中科院分区：
文献类型：
--
作者：
McLean MH;Murray GI;Stewart KN;Norrie G;Mayer C;Hold GL;Thomson J;Fyfe N;Hope M;Mowat NA;Drew JE;El-Omar EM
结直肠癌（CRC）是全世界死亡和发病的主要原因。众所周知，侵袭性结直肠肿瘤基质内的炎症活动是疾病活动的关键预测因素，免疫细胞的类型、密度和位置会影响患者的预后。迄今为止，还没有关于癌前人类结肠腺瘤内炎症表型的报道。评估基质微环境，特别是结直肠肿瘤病变内的炎症活动对于了解早期结直肠癌发生至关重要。通过免疫组织化学评估配对结肠腺瘤和邻近正常结肠粘膜样本中的炎性细胞浸润，并且腺瘤表现出上皮细胞发育不良程度的增加。使用双染色免疫组织化学结合细胞内功能酶的表达来评估巨噬细胞表型。通过 RT-PCR 验证的炎症细胞因子和受体基因的靶向阵列用于评估炎症基因表达。炎症细胞浸润是散发性腺瘤性结肠息肉的一个关键特征，腺瘤性结肠息肉中巨噬细胞、中性粒细胞和 T 细胞（特别是辅助细胞和活化亚群）浸润增加，其增加与高恶性潜能特征相关，即细胞异型增生程度和腺瘤大小的增加。腺瘤内的巨噬细胞表达 iNOS，提示促炎表型。多种炎症细胞因子基因（CXCL1、CXCL2、CXCL3、CCL20、IL8、CCL23、CCL19、CCL21、CCL5）在腺瘤中失调。这项研究提供了癌前结肠腺瘤内炎症增加的证据。这可能有助于确定早期结直肠癌发生和促进的潜在机制途径。
Colorectal cancer (CRC) is a major cause of mortality and morbidity worldwide. Inflammatory activity within the stroma of invasive colorectal tumours is known to be a key predictor of disease activity with type, density and location of immune cells impacting on patient prognosis. To date, there has been no report of inflammatory phenotype within pre-malignant human colonic adenomas. Assessing the stromal microenvironment and particularly, inflammatory activity within colorectal neoplastic lesions is central to understanding early colorectal carcinogenesis. Inflammatory cell infiltrate was assessed by immunohistochemistry in paired colonic adenoma and adjacent normal colonic mucosa samples, and adenomas exhibiting increasing degrees of epithelial cell dysplasia. Macrophage phenotype was assessed using double stain immunohistochemistry incorporating expression of an intracellular enzyme of function. A targeted array of inflammatory cytokine and receptor genes, validated by RT-PCR, was used to assess inflammatory gene expression. Inflammatory cell infiltrates are a key feature of sporadic adenomatous colonic polyps with increased macrophage, neutrophil and T cell (specifically helper and activated subsets) infiltration in adenomatous colonic polyps, that increases in association with characteristics of high malignant potential, namely, increasing degree of cell dysplasia and adenoma size. Macrophages within adenomas express iNOS, suggestive of a pro-inflammatory phenotype. Several inflammatory cytokine genes (CXCL1, CXCL2, CXCL3, CCL20, IL8, CCL23, CCL19, CCL21, CCL5) are dysregulated in adenomas. This study has provided evidence of increased inflammation within pre-malignant colonic adenomas. This may allow potential mechanistic pathways in the initiation and promotion of early colorectal carcinogenesis to be identified.
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影响因子：
3.8
作者：
Hwang, J;Son, KN;Kim, J
通讯作者：
Kim, J
DOI：
10.1084/jem.183.4.1323
发表时间：
1996-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Dinapoli MR;Calderon CL;Lopez DM
通讯作者：
Lopez DM
影响因子：
29.4
作者：
GRISHAM, MB;WARE, K;YAMADA, T
通讯作者：
YAMADA, T
影响因子：
8
作者：
Lin, YM;Furukawa, Y;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
8.8
作者：
Duncan, R.;Carpenter, B.;Main, L. C.;Telfer, C.;Murray, G. I.
通讯作者：
Murray, G. I.