Speciation of selenocompounds by capillary HPLC coupled with ICP-MS using multi-mode gel filtration columns
Speciation of selenocompounds by capillary HPLC coupled with ICP-MS using multi-mode gel filtration columns
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DOI：
10.1039/b412459f
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发表时间：
2005-12
影响因子：
3.4
通讯作者：
Y. Ogra;Kazuo T. Suzuki
中科院分区：
文献类型：
--
作者：
Y. Ogra;Kazuo T. Suzuki
多模式凝胶过滤HPLC柱的毛细管大小耦合电感耦合等离子体质谱进行了评价，以分离天然存在的硒化合物。硒化大蒜中的主要硒化合物，Se-甲基硒代半胱氨酸和γ-谷氨酰-Se-甲基硒代半胱氨酸在较窄的柱（GS 320 A-M5 D，0.5mm i. d.）上分离。× 150 mm）。在硒中毒大鼠尿中发现的硒代谢物，硒糖（1-β-甲基硒基-N-乙酰基-D-半乳糖胺）和三甲基硒也在同一柱上分离。另一方面，硒氨基酸，即，Se-甲基硒代半胱氨酸和硒代甲硫氨酸在较大的柱（GS 320 A-M8 E，0.8mm i. d.× 250 mm）。这些样品不经任何预处理直接引入毛细管柱中。毛细管高效液相色谱分离这些硒化合物到不同的峰与令人满意的灵敏度和更好的S/N比比比传统的高效液相色谱，尽管样品体积减少到1/200（100 nl）。
Multi-mode gel filtration HPLC columns of capillary size coupled with ICP-MS were evaluated as to the separation of naturally occurring selenocompounds. The major selenocompounds found in selenized garlic, i.e., Se-methylselenocysteine and γ-glutamyl-Se-methylselenocysteine, were separated on the narrower column (GS320A-M5D, 0.5 mm i.d. × 150 mm). The selenometabolites found in the urine of a selenium-toxicosis rat, i.e., selenosugar (1-β-methylseleno-N-acetyl-D-galactosamine) and trimethylselenonium, were also separated on the same column. On the other hand, the selenoamino acids, i.e., Se-methylselenocysteine and selenomethionine, were separated on the larger column (GS320A-M8E, 0.8 mm i.d. × 250 mm). These samples were introduced directly into the capillary columns without any pretreatment. The capillary HPLC separated these selenocompounds into distinct peaks with satisfactory sensitivity and a better S/N ratio than conventional HPLC despite that the sample volume was reduced to 1/200 (100 nl).