Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number
Egg-to-embryo transition is driven by differential responses to Ca2+ oscillation number
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DOI：
10.1006/dbio.2002.0788
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发表时间：
2002-10-15
影响因子：
2.7
通讯作者：
Ozil, JP
中科院分区：
文献类型：
--
作者：
Ducibella, T;Huneau, D;Ozil, JP
Ca2+振荡和信号是控制许多细胞事件的基本机制。小鼠卵的激活会引起一系列的Ca2+依赖性事件，包括皮质颗粒胞外分泌，伴随MPF和MAP激酶活性降低的细胞周期恢复，以及母体mrna的募集。其结果是细胞分化的转变，即卵细胞转化为受精卵。通过用实验控制和精确定义的Ca2+瞬态激活小鼠卵，我们证明了这些事件中的每一个都是由不同数量的Ca2+瞬态启动的，而它们的完成需要比它们的启动更多数量的Ca2+瞬态。这种对Ca2+瞬态数量的差异反应的组合提供了强有力的证据，表明单个Ca2+瞬态驱动的信号系统可以启动并驱动细胞进入新的发育途径，并且可以解释与早期发育相关的细胞变化的时间序列。(C) 2002 Elsevier Science （USA）。
Ca2+ oscillations and signaling represent a basic mechanism for controlling many cellular events. Activation of mouse eggs entrains a temporal series of Ca2+-dependent events that include cortical granule exocytosis, cell cycle resumption with concomitant decreases in MPF and MAP kinase activities, and recruitment of maternal mRNAs. The outcome is a switch in cellular differentiation, i.e., the conversion of the egg into the zygote. By activating mouse eggs with experimentally controlled and precisely defined Ca2+ transients, we demonstrate that each of these events is initiated by a different number of Ca2+ transients, while their completion requires a greater number of Ca2+ transients than for their initiation. This combination of differential responses to the number of Ca2+ transients provides strong evidence that a single Ca2+ transient-driven signaling system can initiate and drive a cell into a new developmental pathway, as well as can account for the temporal sequence of cellular changes associated with early development. (C) 2002 Elsevier Science (USA).