Changes in miRNA expressions in the injured small intestine of mice following high-dose radiation exposure
Changes in miRNA expressions in the injured small intestine of mice following high-dose radiation exposure
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DOI：
10.3892/mmr.2020.11054
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发表时间：
2020-06-01
影响因子：
3.4
通讯作者：
Monzen, Satoru
中科院分区：
文献类型：
--
作者：
Chiba, Mitsuru;Uehara, Haruka;Monzen, Satoru
小肠是包括人类在内的哺乳动物体内最具再生性和辐射敏感性的组织之一。暴露在高剂量的电离辐射下会造成严重的肠道损伤。最近，已经进行了几项使用辐射防护剂的研究，以确定减少辐射引起的肠道损伤的方法。然而，彻底了解暴露在高剂量辐射下的小鼠小肠的功能变化对于开发新的和更有效的辐射防护剂是必要的。在本研究中，我们检测了X射线照射(10Gy72小时)后小鼠小肠中microRNA(miRNA/miR)表达的变化。我们利用miRNA微阵列分析在辐射暴露后的小鼠的小肠中发现了7个上调的miRNAs和6个下调的miRNAs。逆转录-定量聚合酶链式反应证实miR-34a-5p高表达。OmicsNet预测叉头盒P1(Foxp1)是miR-34a-5p mRNA的靶标。辐射后小肠Foxp1表达降低，提示Foxp1表达的恢复可能对放射性肠炎有抑制作用。因此，miR-34a-5p是开发新型辐射防护剂的潜在靶向分子。
The small intestine is one of the most highly regenerative and radiosensitive tissues in mammals, including humans. Exposure to high doses of ionizing radiation causes serious intestinal damage. Recently, several investigations have been conducted using radioprotective agents to determine ways for reducing intestinal damage caused by radiation exposure. However, a thorough understanding of functional changes occurring in the small intestine of mice exposed to high-dose radiation is necessary for developing novel and more potent radioprotective agents. In this study, we examined changes in microRNA (miRNA/miR) expressions in the small intestine of mice at 72 h after X-ray exposure (10 Gy). We identified seven upregulated miRNAs and six downregulated miRNAs in the small intestine of mice following radiation exposure using miRNA microarray analysis. Particularly, miR-34a-5p was highly expressed, which was confirmed by reverse transcription-quantitative PCR. Forkhead box P1 (Foxp1) was predicted to be a target of the mRNA of miR-34a-5p using OmicsNet. Decreased Foxp1 expression in the small intestine following radiation exposure was confirmed, suggesting that Foxp1 expression recovery may induce the suppression of radiation-induced enteritis. Therefore, miR-34a-5p is a potential target molecule for developing novel radioprotective agents.