RNA-seq reveals conservation of function among the yolk sacs of human, mouse, and chicken
RNA-seq reveals conservation of function among the yolk sacs of human, mouse, and chicken
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DOI：
10.1073/pnas.1702560114
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发表时间：
2017-06-13
影响因子：
11.1
通讯作者：
Charnock-Jones, D. Stephen
中科院分区：
文献类型：
--
作者：
Cindrova-Davies, Tereza;Jauniaux, Eric;Charnock-Jones, D. Stephen
卵黄囊在系统发育上是最古老的胚外膜。人类胚胎保留卵黄囊，卵黄囊经历了初级和次级发育阶段，但其重要性存在争议。虽然已知它可以合成蛋白质，但它的运输功能被广泛认为是退化的。在这里，我们报告了人类和小鼠卵黄囊的rna测序（RNA-seq）数据，并将这些数据与鸡的数据进行了比较。我们还将人类RNA-seq数据与卵黄囊泡体腔液的蛋白质组学数据联系起来。整个物种转录组的保存表明，人类第二卵黄囊可能在发育早期发挥关键功能，特别是摄取和处理大量和微量营养素，其中许多存在于体腔液中。更广泛地说，我们的发现揭示了产生复杂结构（如胎盘）的进化机制。我们确定了在哺乳动物和鸟类中保守的遗传模块，表明这些模块是核心羊膜遗传库的一部分，是卵生和胎生生殖模式的基石。我们认为，虽然绒毛膜卵黄细胞胎盘从未在人体中建立，但胎盘绒毛、外腔和次级卵黄囊在生理上是等同的。
The yolk sac is phylogenetically the oldest of the extraembryonic membranes. The human embryo retains a yolk sac, which goes through primary and secondary phases of development, but its importance is controversial. Although it is known to synthesize proteins, its transport functions are widely considered vestigial. Here, we report RNA-sequencing (RNA-seq) data for the human and murine yolk sacs and compare those data with data for the chicken. We also relate the human RNA-seq data to proteomic data for the coelomic fluid bathing the yolk sac. Conservation of transcriptomes across the species indicates that the human secondary yolk sac likely performs key functions early in development, particularly uptake and processing of macro-and micronutrients, many of which are found in coelomic fluid. More generally, our findings shed light on evolutionary mechanisms that give rise to complex structures such as the placenta. We identify genetic modules that are conserved across mammals and birds, suggesting these modules are part of the core amniote genetic repertoire and are the building blocks for both oviparous and viviparous reproductive modes. We propose that although a choriovitelline placenta is never established physically in the human, the placental villi, the exocoelomic cavity, and the secondary yolk sac function together as a physiological equivalent.