The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco
The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco
复制标题
玉米晚期胚胎发生丰富蛋白 ZmDHN13 正向调节转基因酵母和烟草的铜耐受性
DOI：
10.1016/j.cj.2018.09.001
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发表时间：
2019-06-01
期刊：
影响因子：
6.6
通讯作者：
Yang, Xinghong
中科院分区：
文献类型：
--
作者：
Liu, Yang;Li, Daxing;Yang, Xinghong
莱亚蛋白在植物种子发育后期积累，在干旱、低温、高温和盐胁迫下表达上调。莱亚蛋白质可根据氨基酸序列分为七组。脱水蛋白属于莱亚蛋白组II。在先前的研究中，ZmDHN13中的玉米KS型转基因烟草增加了对氧化胁迫的耐受性。在本研究中，ZmDHN13在铜胁迫条件下进行了鉴定，然后使用转基因酵母和烟草植物来研究其功能。ZmDHN13结合Cu 2+。它在转基因烟草中的过表达通过结合金属和减少活性氧（ROS）的积累来赋予对铜胁迫的耐受性。三个保守结构域在铜胁迫条件下表现出协同效应。(C)2019中国农业科学院作物科学研究所中国作物学会Elsevier B.V.代表KeAi Communications Co.制作和主持，公司
Late embryogenesis abundant (LEA) proteins accumulate in the late stage of plant seed development, and are upregulated in most plants during drought, cold, heat, or salinity stress. LEA proteins can be classified by amino-acid sequence into seven groups. Dehydrins belong to LEA protein group II. In previous studies, the maize KS type dehydrin ZmDHN13 increased the tolerance of transgenic tobacco to oxidative stress. In the present study, ZmDHN13 was identified under copper stress conditions, and the protein was then characterized using transgenic yeast and tobacco plants to investigate its functions. ZmDHN13 bound Cu2+. Its overexpression in transgenic tobacco conferred tolerance to copper stress by binding metals and reducing the accumulation of reactive oxygen species (ROS). Three conserved domains displayed a cooperative effect under copper stress conditions. (C) 2019 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.