Lipid bilayer microarray for parallel recording of transmembrane ion currents
Lipid bilayer microarray for parallel recording of transmembrane ion currents
复制标题
DOI：
10.1021/ac7016635
复制
发表时间：
2008-01-01
影响因子：
7.4
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Le Pioufle, Bruno;Suzuki, Hiroaki;Takeuchi, Shoji
本文介绍了一种多孔生物芯片，用于同时平行记录离子通过平面脂质双分子层阵列重构的跨膜孔。使用具有微米孔径（phi = 15-50 μ m, t = 20 μ m）的薄聚（对二甲苯）（聚对二甲苯）薄膜，形成高度稳定的双层脂质膜（BLMs），用于跨膜孔的结合；因此，不需要任何技术就可以将大量的blm进行排列。我们在光学上证实了BLM在5 × 5基质中同时形成，在我们的耐久性测试中，BLM持续时间超过15小时。在多个连续的记录位点同时平行记录alamethicin和gramicidin跨膜孔，证明了高通量筛选人工脂质双层中跨膜离子电流的可行性。
This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometersized apertures (phi = 15-50 mu m, t = 20 mu m) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5 x 5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.