Cyanobacterial phycobilisomes. Characterization of the phycobilisomes of Synechococcus sp. 6301.
Cyanobacterial phycobilisomes. Characterization of the phycobilisomes of Synechococcus sp. 6301.
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蓝藻藻胆体。
DOI：
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发表时间：
1978
影响因子：
4.8
通讯作者：
R. Williams
中科院分区：
文献类型：
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作者：
G. Yamanaka;A. Glazer;R. Williams
介绍了一种从单细胞蓝藻聚球藻中制备稳定的藻胆体的方法。6301(也称为Anacystis nidulans)。这些颗粒中的藻蓝蛋白在580 nm处被激发，导致别藻蓝蛋白和别藻蓝蛋白B在673 nm处产生最大荧光发射。电子显微镜显示，藻胆体为杆状簇状结构。棒状物由堆叠的圆盘组成，呈现出主要由藻蓝蛋白(Eiserling，F.A.和Glazer，A.N.(1974)J.超结构)组成的短堆叠的尺寸。决议47，16-25)。在合适的条件下，团簇通过解离成棒状而损失，这与能量传递的损失有关，如荧光发射最大值移动到652 nm所示。聚球藻6301藻胆体除含有有色亚基(携带共价结合的四吡咯修复基团)外，还含有5个非色素多肽。有证据表明，这些无色多肽是藻胆体的真正组成部分。非色素多肽约占藻胆体蛋白质的12%，藻蓝蛋白约占75%，别藻蓝蛋白约占12%。光谱研究表明，藻蓝蛋白在完整的藻胆体中以六聚体形式(αβ)6存在，并且这种聚集体的圆二色和吸光度几乎不受并入藻胆体结构的影响。
A procedure is described for the preparation of stable phycobilisomes from the unicellular cyanobacterium Synechococcus sp. 6301 (also known as Anacystis nidulans). Excitation of the phycocyanin in these particles at 580 nm leads to maximum fluorescence emission, from allophycocyanin and allophycocyanin B, at 673 nm. Electron microscopy shows that the phycobilisomes are clusters of rods. The rods are made up of stacks of discs which exhibit the dimensions of short stacks made up primarily of phycocyanin (Eiserling, F. A., and Glazer, A. N. (1974) J. Ultrastruct. Res. 47, 16-25). Loss of the clusters, by dissociation into rods under suitable conditions, is associated with loss of energy transfer as shown by a shift in fluorescence emission maximum to 652 nm. Synechococcus sp. 6301 phycobilisomes were shown to contain five nonpigmented polypeptides in addition to the colored subunits (which carry the covalently bound tetrapyrrole prosthetic groups) of the phycobiliproteins. Evidence is presented to demonstrate that these colorless polypeptides are genuine components of the phycobilisome. The nonpigmented polypeptides represent approximately 12% of the protein of the phycobilisomes; phycocyanin, approximately 75%, and allophycocyanin, approximately 12%. Spectroscopic studies that phycocyanin is in the hexamer form, (alpha beta)6, in intact phycobilisomes, and that the circular dichroism and absorbance of this aggregate are little affected by incorporation into the phycobilisome structure.