miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner.
miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner.
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DOI：
10.1016/j.bbadis.2015.01.016
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发表时间：
2015-05
期刊：
影响因子：
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通讯作者：
Ying Shi;Guobin Chen;Qingwen Huang;Xu Chen;Jing‐jing Liu;W. Xu;Xiaoxiao Huang;Yun-peng Liu;C. xiao;Deng-Chyang Wu;B. Guleng;Jian‐lin Ren
中科院分区：
文献类型：
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作者：
Ying Shi;Guobin Chen;Qingwen Huang;Xu Chen;Jing‐jing Liu;W. Xu;Xiaoxiao Huang;Yun-peng Liu;C. xiao;Deng-Chyang Wu;B. Guleng;Jian‐lin Ren
三叶因子1（TFF 1）是三叶肽家族的成员，不仅与胃肠道粘膜保护和修复有关，而且与胃肠道肿瘤的发生有关。在早期的研究中，我们进行了序列分析，并确定了一个潜在的miR 423 - 5 p结合位点内的3′-非翻译区的TFF 1使用microRNA靶标预测工具。在目前的研究中，我们证明了TFF 1内的编码DNA区域也是miR 218 - 5 p靶向的候选区域。我们采用实时荧光定量PCR和原位杂交技术分析胃癌组织中miR 218 - 5 p和TFF 1表达的相关性。此外，内源性和外源性TFF 1在胃癌细胞中被miR 218 - 5 p抑制，并以Erk 1/2依赖的方式影响GC的进展。靶向miR 218 - 5 p可能为GC的治疗提供新的策略。
Trefoil factor 1 (TFF1), a member of the trefoil peptide family, is not only associated with mucosal protection and restoration but is also correlated with tumorigenesis of the gastrointestinal tract. In an early study, we performed sequence analysis and identified one potential miR423-5p binding site within the 3′-untranslated region of TFF1 using microRNA target prediction tools. In the current study, we demonstrated that the coding DNA region within TFF1 is also a candidate for miR218-5p targeting. We used real-time PCR andin situhybridization to analyze the correlation between miR218-5p and TFF1 expression in tumor lesions and paracancerous tissue in gastric cancer (GC) samples. Additionally, endogenous and exogenous TFF1 were suppressed by miR218-5p in gastric cancer cells and influenced the progression of GC in an Erk1/2-dependent manner. Targeting miR218-5p may provide a novel strategy for the treatment of GC.