Construction of Vascularized Tissue Engineered Bone with nHA-Coated BCP Bioceramics Loaded with Peripheral Blood-Derived MSC and EPC to Repair Large Segmental Femoral Bone Defect.
Construction of Vascularized Tissue Engineered Bone with nHA-Coated BCP Bioceramics Loaded with Peripheral Blood-Derived MSC and EPC to Repair Large Segmental Femoral Bone Defect.
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DOI：
10.1021/acsami.2c15000
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发表时间：
2022-12
影响因子：
9.5
通讯作者：
Huihui Wang;Xiangfeng Li;Sike Lai;Q. Cao;Yunyi Liu;Jian Li;Xiangdong Zhu;W. Fu;Xing‐dong Zhang
中科院分区：
文献类型：
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作者：
Huihui Wang;Xiangfeng Li;Sike Lai;Q. Cao;Yunyi Liu;Jian Li;Xiangdong Zhu;W. Fu;Xing‐dong Zhang
节段性骨缺损的再生修复是骨科领域亟待解决的问题。支架植入后快速诱导血管生成和成骨活性至关重要。本研究将外周血间充质干细胞(PBMSC)和内皮祖细胞(PBEPC)混合的独特组织工程策略应用于三维打印的纳米羟基磷灰石(NHA/BCP)涂层的双相磷酸钙(BCP)支架中，构建了一种用于兔股骨SBD修复的新型血管化组织工程骨(VTEB)。PBMSC与PBEPC的2D共培养显示，两者均能促进细胞向成骨或血管分化，尤其是在PBEPC/PBMSC=75：25的情况下。3d共培养结果表明，纳米羟基磷灰石涂层可进一步促进PBEPC/PBMSC在BCP支架上的黏附、增殖以及向成骨和血管分化。体内实验表明，在BCP、BCP-PBEPC/PBMSC、NHA/BCP、NHA/BCP-PBEPC/PBMSC四种材料中，NHA/BCP-PBEPC/PBMSC组诱导的血管形成和新骨形成最好，修复良好。揭示了纳米羟基磷灰石和PBEPC/PBMSC对BCP支架的血管生成和成骨的协同作用。因此，本研究中VTEB的构建为SBD的再生修复提供了可能。
The regenerative repair of segmental bone defect (SBD) is an urgent problem in the field of orthopedics. Rapid induction of angiogenesis and osteoinductivity after implantation of scaffold is critical. In this study, a unique tissue engineering strategy with mixture of peripheral blood-derived mesenchymal stem cells (PBMSC) and endothelial progenitor cells (PBEPC) was applied in a 3D-printed biphasic calcium phosphate (BCP) scaffold with highly bioactive nano hydroxyapatite (nHA) coating (nHA/BCP) to construct a novel vascularized tissue engineered bone (VTEB) for rabbit femoral SBD repair. The 2D coculture of PBMSC and PBEPC showed that they could promote the osteogenic or angiogenic differentiation of the cells from each other, especially in the group of PBEPC/PBMSC = 75:25. Besides, the 3D coculture results exhibited that the nHA coating could further promote PBEPC/PBMSC adhesion, proliferation, and osteogenic and angiogenic differentiation on the BCP scaffold. In vivo experiments showed that among the four groups (BCP, BCP-PBEPC/PBMSC, nHA/BCP, and nHA/BCP-PBEPC/PBMSC), the nHA/BCP-PBEPC/PBMSC group induced the best formation of blood vessels and new bone and, thus, the good repair of SBD. It revealed the synergistic effect of nHA and PBEPC/PBMSC on the angiogenesis and osteogenesis of the BCP scaffold. Therefore, the construction of VTEB in this study could provide a possibility for the regenerative repair of SBD.