The direct application of plasma droplet digital PCR in the ultra-early pathogen detection and warning during sepsis: Case reports
The direct application of plasma droplet digital PCR in the ultra-early pathogen detection and warning during sepsis: Case reports
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DOI：
10.1016/j.jiph.2022.03.008
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发表时间：
2022-03-26
影响因子：
6.7
通讯作者：
Zhang, Wen-Hong
中科院分区：
文献类型：
--
作者：
Lin, Ke;Zhang, Hao-Cheng;Zhang, Wen-Hong
背景：脓毒症是一种严重的急性疾病，死亡率高，对于存活者，长期发病。方法：采用液滴数字PCR（ddPCR）技术对2例脓毒症患者进行检测。结果：ddPCR对脓毒症的超早期诊断及指导用药具有重要的临床价值。结论：ddPCR具有灵敏度高、周转时间短等优点，可在脓毒症的超早期进行快速、准确的诊断，有利于脓毒症的及时、最佳治疗。(c)2022由Elsevier Ltd代表沙特国王本阿卜杜勒阿齐兹健康科学大学出版。CC_BY_NC_ND_4.0
Background: Sepsis is a severe and acute disease associated with high mortality, for those who survive, long-term morbidity. In-time detection of pathogen for proper and optimized treatment in the early stage of sepsis is crucial.Methods: We performed droplet digital PCR (ddPCR) on two cases of sepsis. The clinical information, laboratory test results, and the therapeutic regimen was detailed recorded.Results: ddPCR showed its clinical value of sepsis ultra-early diagnosis and the guidance of medication in both cases we provided. Conclusions: ddPCR has potential for rapid and precise diagnose in ultra-early stage of sepsis for its high sensitivity and short turn-around-time, thus benefiting in optimal and timely treatment of sepsis.(c) 2022 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences. CC_BY_NC_ND_4.0