Cloning and characterization of a specific interleukin (IL)-13 binding protein structurally related to the IL-5 receptor alpha chain
Cloning and characterization of a specific interleukin (IL)-13 binding protein structurally related to the IL-5 receptor alpha chain
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DOI：
10.1074/jbc.271.28.16921
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发表时间：
1996-07-12
影响因子：
4.8
通讯作者：
Ferrara, P
中科院分区：
文献类型：
--
作者：
Caput, D;Laurent, P;Ferrara, P
白细胞介素 13 (IL-13) 是一种由活化的 T 淋巴细胞分泌的细胞因子，与 IL-4 具有许多（但不是全部）生物活性。这些重叠的活性可能是由于存在共同的受体成分，两种蛋白被描述为 IL-4 受体的成分，类似于 140-kDa 糖蛋白 (IL-4R) 和 IL-2 受体的伽马链 (gamma c)，但这些蛋白都不结合 IL-13。我们从 Caki-1 人肾癌细胞系中克隆了编码 IL-13 结合蛋白 (IL-13R) 的 cDNA。克隆的 cDNA 编码 380 个氨基酸的蛋白质，具有造血细胞因子受体家族特征的两种共有模式和短胞质尾。IL-13R 显示与 IL-5 受体的同源性，并在较小程度上与催乳素受体同源，用 IL-13R cDNA 转染的 COS-7 细胞以高亲和力结合 IL-13，但不结合 IL-4，与克隆的 cDNA 共转染的 COS-7 细胞IL-13R cDNA 和 IL-CR cDNA 导致少量识别 IL-4 和 IL-13 的受体重建。逆转录聚合酶链反应分析仅在已知结合 IL-13 的细胞系中检测到受体转录物。
Interleukin-13 (IL-13) is a cytokine secreted by activated T lymphocytes that shares many, but not all, biological activities with IL-4. These overlapping activities are probably due to the existence of common receptor components, Two proteins have been described as constituents of the IL-4 receptor, a similar to 140-kDa glycoprotein (IL-4R) and the gamma chain (gamma c) of the IL-2 receptor, but neither of these proteins binds IL-13, We have cloned a cDNA encoding an IL-13 binding protein (IL-13R) from the Caki-1 human renal carcinoma cell line. The cloned cDNA encodes a 380-amino acid protein with two consensus patterns characteristic of the hematopoietic cytokine receptor family and a short cytoplasmic tail, The IL-13R shows homology with the IL-5 receptor, and to a lesser extent, with the prolactin receptor, COS-7 cells transfected with the IL-13R cDNA bind IL-13 with high affinity but do not bind IL-4, COS-7 cells co transfected with the cloned IL-13R cDNA and IL-CR cDNA resulted in the reconstitution of a small number of receptors that recognized both IL-4 and IL-13, Reverse transcription-polymerase chain reaction analysis detected the receptor transcript only in cell lines known to bind IL-13.