The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
The Ralstonia solanacearum effector RipI induces a defence reaction by interacting with the bHLH93 transcription factor in Nicotiana benthamiana
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Ralstonia solanacearum 效应子 RipI 通过与本塞姆氏烟草中的 bHLH93 转录因子相互作用诱导防御反应
DOI：
10.1111/mpp.12937
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发表时间：
2020
影响因子：
4.9
通讯作者：
Zou Huasong
中科院分区：
文献类型：
--
作者：
Zhuo Tao;Wang Xue;Chen Zhengyu;Cui Haitao;Zeng Yanhong;Chen Yang;Fan Xiaojing;Hu Xun;Zou Huasong
摘要青枯雷尔氏菌向植物细胞释放一系列的效应物，改变寄主防御反应。效应蛋白RIPI在感染过程中的作用尚未阐明。在这项研究中，我们证明了Ripi的瞬时过表达诱导了烟草的过敏反应(HR)，上调了HR标记基因HIN1。青枯菌Ripi基因的缺失导致番茄(Solanum Lycopsicum)植株的致病力增强。通过酵母双杂交和Pull-down实验，我们确定了N.benthamiana转录因子bHLH93和Ripi之间的相互作用，这两个基因都可以定位在拟南芥原生质体的细胞核中。沉默bHLH93可显著抑制RIPI诱导的HR和PDF1.2防御基因的表达。这些数据表明，青枯病菌效应子Ripi通过与bHLH93转录因子相互作用而诱导宿主防御反应。
Abstract Ralstonia solanacearum releases a set of effectors into plant cells that modify the host defence reaction. The role of the effector protein RipI during infection has not been elucidated. In this study, we demonstrated that transient overexpression of RipI induces the hypersensitive response (HR), up‐regulating the HR marker gene hin1, in Nicotiana benthamiana. Deletion of R. solanacearum ripI led to increased virulence in tomato (Solanum lycopersicum) plants. Through yeast two‐hybrid and pull‐down assays, we identified an interaction between the N. benthamiana transcription factor bHLH93 and RipI, both of which could be localized in the nucleus of Arabidopsis protoplasts. Silencing of bHLH93 markedly attenuated the RipI‐induced HR and induced expression of the PDF1.2 defence gene. These data demonstrate that the R. solanacearum effector RipI induces a host defence reaction by interacting with the bHLH93 transcription factor.