Development of Ochratoxin Aptasensor Based on DNA Metal Nanoclusters
Development of Ochratoxin Aptasensor Based on DNA Metal Nanoclusters
复制标题
基于DNA金属纳米团簇的赭曲霉毒素适体传感器的研制
DOI：
10.1166/nnl.2019.2981
复制
发表时间：
2019-08-01
影响因子：
--
通讯作者：
Yang, Yunhui
中科院分区：
文献类型：
--
作者：
Gu, Mengqiao;Liu, Jiali;Yang, Yunhui
本文基于OTA和OTA适配子(DNA2)的特异性，构建了一种信号关闭适配子传感器，用于检测小分子赭曲霉毒素A(OTA)。首次将3‘端修饰有巯基的DNA1链固定在金电极上，与DNA2杂交形成双链。以DNA2末端修饰的胸腺嘧啶T为模板，合成了能催化过氧化氢的模拟过氧化物酶的纳米铜化合物。在OTA的存在下，它与DNA2特异结合，使DNA2解离，CuNCS也是如此。溶液中OTA越多，核酸适体和铜纳米碳链从金电极上解离的越多，电极上残留的铜纳米团簇就越少，计时电流法测得的电极电流响应值越小。从而实现了OTA的定量测定。该适体传感器为红葡萄酒样品中OTA的检测提供了一种简单、快速的技术。
In this paper, a signal-off aptasensor was constructed based on the specificity of OTA and OTA aptamer (DNA2), it can detect the small molecule ochratoxin A (OTA). DNA1 chain with mercapto modified at 3'-end was firstly immobilized on gold electrode, which cross-fertilize with DNA 2 to form double strands. 30 thymine T modified at the end of DNA2 were used as templates to synthesize Cu NCs which is peroxidase mimics and can catalyze hydrogen peroxide. Presence of OTA, it specifically binds to DNA2, which made DNA2 dissociated, and so did Cu NCs. The more OTA in solution, the more the aptamers and Cu NCs dissociated from the gold electrode, the less copper nanoclusters remained on the electrode, and the smaller the current response value of the electrode measured by chronoamperometry. Thus, the quantitative determination of OTA can be achieved. This aptasensor provides a simple and fast technique for OTA detection in red wine samples.