Protein-free culture medium containing polyvinylalcohol, vitamins, and amino acids supports development of eight-cell hamster embryos to hatching blastocysts.
Protein-free culture medium containing polyvinylalcohol, vitamins, and amino acids supports development of eight-cell hamster embryos to hatching blastocysts.
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含有聚乙烯醇、维生素和氨基酸的无蛋白培养基支持八细胞仓鼠胚胎发育至孵化囊胚。
DOI：
10.1002/jez.1402470211
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Bavister,BD
中科院分区：
文献类型：
--
作者：
Kane,MT;Bavister,BD
八细胞仓鼠胚胎的体外发育需要在培养基中添加氨基酸和一组水溶性维生素。本文研究了不同类型的大分子对囊胚孵化及维生素需要量的影响。胚胎在含和不含维生素的合成高分子聚乙烯醇（PVA）和不同类型的牛血清白蛋白（BSA）存在下培养3天。结果表明，在含有维生素和氨基酸的培养基中，PVA是唯一的大分子，8细胞胚胎发育为孵化囊胚。某些类型的BSA的存在减少了但并没有消除对维生素的需求。在支持囊胚孵化方面，谷氨酰胺单独与氨基酸补充剂一样有效。这些结果首次证明8细胞仓鼠胚胎可以在化学定义的培养基中培养成孵化囊胚。
In vitro development of eight‐cell hamster embryos to hatching blastocysts requires the presence of amino acids and a group of water‐soluble vitamins in the culture medium. The present studies investigated the effect of type of macromolecule on blastocyst hatching and on the requirement for vitamins. Embryos were cultured for 3 days in the presence of the synthetic macromolecule polyvinylalcohol (PVA) and of different types of bovine serum albumin (BSA), both with and without vitamins. The results showed that eight‐cell embryos develop to hatching blastocysts in the presence of vitamins and amino acids with PVA as the only macromolecule in the medium. The presence of certain types of BSA reduced but did not eliminate the need for vitamins. Glutamine alone was as efficient as a complete amino acid supplement in supporting blastocyst hatching. These results demonstrate for the first time that eight‐cell hamster embryos can be cultured to hatching blastocysts in a chemically defined medium.