High-Throughput Platform for Rapid Deployment of Antimicrobial Agents
High-Throughput Platform for Rapid Deployment of Antimicrobial Agents
复制标题
DOI：
10.1021/co200088c
复制
发表时间：
2011-11-01
影响因子：
--
通讯作者：
Gonzalez-Moa, Maria J.
中科院分区：
文献类型：
--
作者：
Svarovsky, Sergei A.;Gonzalez-Moa, Maria J.
介绍了一种利用可寻址的高密度随机序列肽微阵列进行细菌结合试验的新方法。当细菌结合在竞争过量的相应细菌脂多糖（LPS）存在下进行时，大多数观察到的细菌结合被抑制，表明LPS是细菌结合肽的主要靶标。重要的是，所选肽的氨基酸组成与天然抗微生物肽的组成非常相似。所选肽与多价纳米颗粒支架的缀合产生显示出有效的抗菌凝集活性的构建体。该系统足够通用，可以潜在地产生针对几乎任何病原体的抗菌剂。
A new approach to conducting bacterial binding assays by using an addressable high density random sequence peptide microarray is described. When bacterial binding is carried out in the presence of a competing excess of corresponding bacterial lipopolysaccharide (LPS), most of the observed bacterial binding is inhibited, suggesting that LPS is the major target of the bacterial binding peptides. Importantly, the amino acid composition of the selected peptides closely resembles the composition of natural antimicrobial peptides. Conjugation of selected peptides to polyvalent nanoparticle scaffold yields constructs that show potent antibacterial agglutination activities. The system is general enough to potentially create antimicrobial agents to virtually any pathogen.