Antiviral effect of prodigiosin isolated from fish intestinal bacteria against Micropterus salmoides rhabdovirus
Antiviral effect of prodigiosin isolated from fish intestinal bacteria against Micropterus salmoides rhabdovirus
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DOI：
10.1016/j.aquaculture.2023.739683
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发表时间：
2023-05-27
期刊：
影响因子：
4.5
通讯作者：
Wang，Gaoxue
中科院分区：
文献类型：
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作者：
Song，Kaige;Li，Jing;Wang，Gaoxue
大口黑鲈弹状病毒（Micropterus salmoidesrhabdovirus，MSRV）是大口黑鲈养殖中最具杀伤力的病毒病原，对黑鲈鱼苗的健康和生存构成严重威胁。然而，仍然没有具体的商业化药物或疫苗来控制MSRV感染。在这项研究中，我们确定了一个红色的细菌菌株从肠道的M。salmoides、粘质沙雷氏菌MS 01。有趣的是，我们发现沉积物的甲醇提取物（SED）的S。marcescensMS 01具有高效的抗MSRV活性。当浓度为30 mg/L时，SED能有效降低MSRV的毒力，对MSRV糖蛋白和核蛋白表达的抑制率达99%。此外，SED还能抑制MSRV诱导的氧化应激，提高SOD和CAT中抗氧化酶的活性。进一步纯化、活性分析和多光谱鉴定表明灵菌红素在SED中对MSRV的抑制起关键作用。总之，我们的研究结果表明，SED或灵菌红素有可能作为一个有前途的抗MSRV在水产养殖场的抗病毒策略是有用的。
Micropterus salmoidesrhabdovirus (MSRV) is the most lethal viral pathogen in largemouth bass farming, especially threatening the health and survival of bass fry. However, there are still no specific commercialized drugs or vaccines to control MSRV infection. In this study, we identified a red-pigmented bacterial strain from the gut ofM. salmoides,Serratia marcescensMS01. Interestingly, we found the methanol extracts of sediment (SED) ofS. marcescensMS01 showed highly efficient anti-MSRV activity. SED effectively reduced the virulence of MSRV at a concentration of 30 mg/L, while the inhibition rate of glycoprotein and nucleoprotein expression of MSRV reached 99% in GCO cells. Besides, SED inhibited MSRV-induced oxidative stress and increased the activity of antioxidant enzyme in SOD and CAT. Subsequently, further purification, activity analysis, and multi-spectroscopic identification indicated prodigiosin played a crucial role in inhibiting MSRV in SED. Altogether, our finding suggests that SED or prodigiosin has the potential to be useful as a promising antiviral strategy against MSRV in aquaculture farms.