Dietary fibers stimulate colonic cell proliferation by different mechanisms at different sites.
Dietary fibers stimulate colonic cell proliferation by different mechanisms at different sites.
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DOI：
10.1080/01635589409514352
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. Zhang;J. Lupton
中科院分区：
文献类型：
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作者：
J. Zhang;J. Lupton
在60只雄性Sprague-Dawley大鼠中研究了膳食纤维类型（纤维素、果胶或燕麦麸）对结肠短链脂肪酸（SCFA）、管腔pH值和上皮细胞增殖的影响。通过[3 H]胸苷掺入DNA来测量体内细胞增殖，测量体内pH，并通过气相色谱法测定管腔SCFA。果胶和燕麦麸皮饮食产生更高的浓度的所有类型的SCFA和总SCFA比纤维素在每个网站。SCFA浓度越高，每个位点的pH值越低。在盲肠中，SCFA浓度与每个隐窝柱的细胞数（r = 0.961）、每个隐窝的总细胞数（r = 0.963）和增殖区（r = 0.845）正相关。相反，在远端结肠中，SCFA浓度和细胞增殖测量值之间没有显著相关性。这些数据表明，纤维刺激盲肠细胞增殖，通过生产SCFA。纤维对远端结肠炎的影响可能通过不同的机制介导。
The effect of dietary fiber type (cellulose, pectin, or oat bran) on colonic short-chain fatty acids (SCFA), luminal pH, and epithelial cell proliferation was examined in 60 male Sprague-Dawley rats. In vivo cell proliferation was measured by [3H]thymidine incorporation into DNA, pH was measured in vivo, and luminal SCFA were determined by gas chromatography. The pectin and oat bran diets produced higher concentrations of all types of SCFA and total SCFA than cellulose at every site. The higher the SCFA concentration, the lower was the pH at each site. In the cecum, the concentration of SCFA was positively associated with the number of cells per crypt column (r = 0.961), total cells per crypt (r = 0.963), and the proliferative zone (r = 0.845). In contrast, in the distal colon, there was no significant correlation between SCFA concentration and measurements of cell proliferation. These data suggest that fiber stimulates cecal cell proliferation through the production of SCFA. The effects of fiber on distal colonic cytokinetics are likely mediated through a different mechanism.