SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc
SLC39A14, a LZT protein, is induced in adipogenesis and transports zinc
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DOI：
10.1111/j.1742-4658.2005.04580.x
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发表时间：
2005-04-01
期刊：
影响因子：
5.4
通讯作者：
Imagawa, M
中科院分区：
文献类型：
--
作者：
Tominaga, K;Kagata, T;Imagawa, M
在脂肪细胞分化过程中，有一系列复杂的基因表达。我们之前已经分离到许多基因，它们的表达水平通过向小鼠3T3-L1前脂肪细胞添加诱导剂而迅速上升。本文报道了ZIP转运蛋白亚家族中LZT蛋白成员SLC39A14的分离和鉴定。在分化的早期阶段，SLC39A14基因的表达被强烈而迅速地诱导。此外，它高度受3T3-L1细胞潜在分化状态的限制，在非成脂NIH-3T3细胞中的表达水平很低，表明其在脂肪细胞分化中具有优势表达。锌摄取实验表明SLC39A14具有锌转运蛋白的功能。综上所述，这些结果表明SLC39A14在脂肪形成的早期阶段起着锌转运蛋白的作用。
During adipocyte differentiation, there is an underlying complex series of gene expressions. We have previously isolated many genes whose expression levels are quickly elevated by the addition of inducers to mouse 3T3-L1 preadipocyte cells. Here we report the isolation and characterization of SLC39A14, a member of the LZT proteins, one of the subfamilies of ZIP transporters. The expression of the SLC39A14 gene was strongly and rapidly induced at the early stages of differentiation. Moreover, it was highly restricted to the potential differentiation state of 3T3-L1 cells and the expression level was quite low in the nonadipogenic NIH-3T3 cells, indicating a dominant expression in adipocyte differentiation. The zinc uptake assay revealed that SLC39A14 functions as a zinc transporter. Taken together, these results suggest that SLC39A14 plays a role as a zinc transporter during the early stages of adipogenesis.