Night Monkey Hybrids Exhibit De Novo Genomic and Karyotypic Alterations: The First Such Case in Primates.
Night Monkey Hybrids Exhibit De Novo Genomic and Karyotypic Alterations: The First Such Case in Primates.
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DOI：
10.1093/gbe/evx058
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发表时间：
2017-04-01
影响因子：
3.3
通讯作者：
Koga A
中科院分区：
文献类型：
--
作者：
Hirai H;Hirai Y;Morimoto M;Kaneko A;Kamanaka Y;Koga A
利用分子染色体分析，我们发现夜猴杂交产生的圈养交配之间的女性Aotus azarae boliviensis（2n = 50）和男性Aotus lemurinus griseimembra（2n = 53）。这对父母总共生下了七个后代，包括一个雄性和六个雌性--这一模式与哈勒的规则一致。对四个杂交后代进行了染色体研究。其中两个表现出相对“简单”的混合核型，包括不同的染色体数目（2n = 51，52），这是由于在父亲（n = 26，27）的异形常染色体对形成的。另外两只杂交猴表现出从头基因组和核型改变。详细的分析表明，一个个体携带两个亲本物种的混合核型和X染色体三体（53，XXX）。第二个个体显示18号染色体三体（52，XX，+18）和21和23号常染色体之间的相互易位（52，XX，+18，t（21;23））。有趣的是，第二只猴子在血细胞中表现出嵌合体（mos 52，XX，+18[87]/52，XX，+18，t（21;23）[85]），但在皮肤成纤维细胞中只有单一核型（52，XX，+18）。X-和18-三体来自于早期胚胎细胞分裂中母亲染色体的加倍，考虑到它只在血细胞中观察到，相互易位可能在后代的骨髓中发展。这种在杂种个体中发生的三体现象是胎盘哺乳动物中的独特发现。
Using molecular chromosomal analyses, we discovered night monkey hybrids produced in captivity from matings between a female Aotus azarae boliviensis (2n = 50) and a male Aotus lemurinus griseimembra (2n = 53). The parents produced seven offspring in total, including one male and six females—a pattern consistent with Haldane’s rule. Chromosomal studies were conducted on four of the hybrid offspring. Two of them showed relatively “simple” mixture karyotypes, including different chromosome numbers (2n = 51, 52), which were formed because of a heteromorphic autosome pair in the father (n = 26, 27). The other two hybrid monkeys exhibited de novo genomic and karyotypic alterations. Detailed analysis of the alterations revealed that one individual carried a mixture karyotype of the two parental species and an X chromosome trisomy (53,XXX). The second individual displayed trisomy of chromosome 18 (52,XX,+18) and a reciprocal translocation between autosomes 21 and 23 (52,XX,+18,t(21;23)). Interestingly, the second monkey exhibited mosaicism among blood cells (mos52,XX,+18[87]/52,XX,+18,t(21;23)[85]), but only a single karyotype (52,XX,+18) in skin fibroblast cells. The X- and 18-trisomies were derived from a doubling of the mother’s chromosomes in early embryonic cell division, and the reciprocal translocation likely developed in the bone marrow of the offspring, considering that it was observed only in blood cells. Such occurrence of trisomies in hybrid individuals is a unique finding in placental mammals.