POLYMERASE CHAIN REACTION-GENE PROBE DETECTION OF MICROORGANISMS BY USING FILTER-CONCENTRATED SAMPLES
POLYMERASE CHAIN REACTION-GENE PROBE DETECTION OF MICROORGANISMS BY USING FILTER-CONCENTRATED SAMPLES
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DOI：
10.1128/aem.57.12.3529-3534.1991
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发表时间：
1991-12-01
影响因子：
4.4
通讯作者：
ATLAS, RM
中科院分区：
文献类型：
--
作者：
BEJ, AK;MAHBUBANI, MH;ATLAS, RM
为了利用聚合酶链式反应基因探针检测环境样品中的低水平微生物，对样品进行了过滤浓缩。荧光团(微孔公司)过滤器与PCRDNA扩增相兼容，而其他各种过滤器，包括硝酸纤维和醋酸纤维过滤器，则抑制了PCR扩增。通过将细胞集中在荧光孔过滤器上，并通过冻融循环释放DNA，可以在不拆卸过滤器的情况下进行PCRDNA扩增。用荧光团FHLP和FGLP过滤器进行浓缩，可以用聚合酶链式反应基因探针检测100毫升样品中的单个微生物细胞。
To detect low levels of microorganisms in environmental samples by using polymerase chain reaction (PCR)-gene probe detection, samples were concentrated by filtration. Fluoropore (Millipore Corp.) filters were compatible with PCR DNA amplification, whereas various other filters including nitrocellulose and cellulose acetate filters inhibited PCR amplification. By concentrating cells on Fluoropore filters and releasing the DNA by freeze-thaw cycling, PCR DNA amplification could be performed without removing the filter. Concentration with Fluoropore FHLP and FGLP filters permitted the detection of single cells of microorganisms in 100-ml samples by PCR-gene probes.