Intensity Enhanced Cerenkov Luminescence Imaging Using Terbium-Doped Gd2O2S Microparticles
Intensity Enhanced Cerenkov Luminescence Imaging Using Terbium-Doped Gd2O2S Microparticles
复制标题
使用掺铽 Gd2O2S 微粒进行强度增强切伦科夫发光成像。
DOI：
10.1021/acsami.5b00432
复制
发表时间：
2015-06-10
影响因子：
9.5
通讯作者：
Tian, Jie
中科院分区：
文献类型：
--
作者：
Cao, Xin;Chen, Xueli;Tian, Jie
强度弱和穿透深度差是制约切伦科夫发光成像（CLI）临床应用的两大障碍。在这项概念验证研究中，我们通过使用镧系元素的放射性发光微粒（RLMP）克服了这些限制，称为铽掺杂的Gd2O2S。表征实验表明，由切伦科夫发光激发的发射可以忽略不计，而光谱实验表明，RLMP实际上可以被γ射线激发。一系列的体外实验表明，RLMPs显着提高的强度和穿透能力的CLI，已扩展到深至15毫米。在体内假瘤研究进一步证明了巨大的潜力，这种增强策略的切伦科夫发光成像在活体动物研究。
Weak intensity and poor penetration depth are two big obstacles toward clinical use of Cerenkov luminescence imaging (CLI). In this proof-of-concept study, we overcame these limitations by using lanthanides-based radioluminescent microparticles (RLMPs), called terbium doped Gd2O2S. The characterization experiment showed that the emission excited by Cerenkov luminescence can be neglected whereas the spectrum experiment demonstrated that the RLMPs can actually be excited by γ-rays. A series of in vitro experiments demonstrated that RLMPs significantly improve the intensity and the penetration capacity of CLI, which has been extended to as deep as 15 mm. In vivo pseudotumor study further prove the huge potential of this enhancement strategy for Cerenkov luminescence imaging in living animal studies.