Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats
Contraction of the type I IFN locus and unusual constitutive expression of IFN-α in bats
复制标题
DOI：
10.1073/pnas.1518240113
复制
发表时间：
2016-03-08
影响因子：
11.1
通讯作者：
Baker, Michelle L.
中科院分区：
文献类型：
--
作者：
Zhou, Peng;Tachedjian, Mary;Baker, Michelle L.
蝙蝠携带许多新出现和重新出现的病毒，其中一些在其他哺乳动物中具有高致病性，但在蝙蝠中不会引起疾病的临床症状。为了确定干扰素（IFN）在蝙蝠与病毒共存的能力中的作用，我们对澳大利亚黑狐蝠（Pteropus alecto）的I型IFN基因座进行了测序，据我们所知，这是任何蝙蝠物种IFN区域的第一个基因图谱。我们的研究结果揭示了一个高度收缩的I型IFN家族，仅由10个IFN组成，包括三个功能性IFN-α基因座。此外，三种IFN-α基因在未刺激的蝙蝠组织和细胞中组成型表达，并且它们的表达不受病毒感染的影响。组成性表达的IFN-α导致诱导与抗病毒活性和抗DNA损伤相关的IFN刺激基因的子集，为可能与蝙蝠与病毒共存的能力有关的独特IFN系统提供证据。
Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs of disease in bats. To determine the role of interferons (IFNs) in the ability of bats to coexist with viruses, we sequenced the type I IFN locus of the Australian black flying fox, Pteropus alecto, providing what is, to our knowledge, the first gene map of the IFN region of any bat species. Our results reveal a highly contracted type I IFN family consisting of only 10 IFNs, including three functional IFN-alpha loci. Furthermore, the three IFN-alpha genes are constitutively expressed in unstimulated bat tissues and cells and their expression is unaffected by viral infection. Constitutively expressed IFN-alpha results in the induction of a subset of IFN-stimulated genes associated with antiviral activity and resistance to DNA damage, providing evidence for a unique IFN system that may be linked to the ability of bats to coexist with viruses.