JUVENILE-HORMONE BINDING-PROTEIN IN HEMOLYMPH OF MANDUCA-SEXTA JOHANNSON (LEPIDOPTERA-SPHINGIDAE)
JUVENILE-HORMONE BINDING-PROTEIN IN HEMOLYMPH OF MANDUCA-SEXTA JOHANNSON (LEPIDOPTERA-SPHINGIDAE)
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DOI：
10.1073/pnas.71.2.493
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发表时间：
1974-01-01
影响因子：
11.1
通讯作者：
LAW, JH
中科院分区：
文献类型：
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作者：
KRAMER, KJ;SANBURG, LL;LAW, JH
C18：幼激素极易溶于水，单体溶液大于10-5M。通过凝胶渗透色谱法和圆盘凝胶电泳法，在体内注射或向体外血淋巴中加入水相幼体激素，可发现幼体激素与蛋白质的络合作用。该蛋白的表观分子量为3.4 × 104，以微摩尔浓度存在于血淋巴中。激素与蛋白质的结合可以描述为一个简单的热力学平衡，解离常数为3 × 10-7M，蛋白质对激素的亲和力远高于对水解产物的亲和力。
C18:juvenile hormone is quite soluble in water, yielding a monomeric solution greater than 10-5M.In vivoinjection or addition of aqueous juvenile hormone to the hemolymphin vitroshows the complexation of juvenile hormone to a protein, as demonstrated by gel permeation chromatography and disc-gel electrophoresis. The protein has an apparent molecular weight of 3.4 × 104and is present in the hemolymph at a concentration in the micromolar range. The binding of the hormone to the protein can be described as a simple thermodynamic equilibrium with a dissociation constant of 3 × 10-7M, and the protein has a much higher affinity for the hormone than for the hydrolysis products.