Immunocytochemical localization of human intrinsic factor: the nonstimulated stomach.
Immunocytochemical localization of human intrinsic factor: the nonstimulated stomach.
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人内在因子的免疫细胞化学定位：未刺激的胃。
DOI：
10.1016/0016-5085(80)90374-1
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发表时间：
1980
期刊：
影响因子：
29.4
通讯作者：
R. Allen
中科院分区：
文献类型：
--
作者：
J. Levine;P. Nakane;R. Allen
免疫荧光法在光镜水平上发现人固有因子(IF)存在于壁细胞表面，但没有直接证据表明IF是在壁细胞中合成和分泌的。为了提供这一证据，利用免疫化学单特异性抗体对纯化的人IF进行定位于人胃的超微结构水平。我们定义了最佳的免疫细胞化学条件，并在4名禁食受试者的眼底活检中确定了IF的亚细胞位置。内源性因子存在于壁细胞的核周膜、粗面内质网、高尔基体、小管泡和多囊体膜上。细胞核、线粒体、基侧表面膜、钝的微绒毛和胞浆中缺少内源性因子。因此，我们将壁细胞定义为IF合成的位置。所观察到的IF在细胞内的分布表明，染色的膜结构之间存在相互关系，IF的分泌可能依赖于膜的移位。进一步的免疫细胞化学和生理学研究将增加我们对IF分泌和壁细胞的细胞生理学的了解。
Human intrinsic factor (IF) is found on the surface of parietal cells at the light microscopic level by immunofluorescence, but there is no direct evidence that IF is synthesized in and secreted from this cell. In order to provide this evidence, IF was localized at the ultrastructural level in human stomach, using an immunochemically monospecific antibody against purified human IF. We defined optimal immunocytochemical conditions and identified the subcellular location of IF in fundic biopsies from 4 fasting subjects. Intrinsic factor was found on the perinuclear membrane, on rough endoplasmic reticulum, Golgi apparatus, tubulovesicles, and membranes of the multivesicular body in parietal cells. Intrinsic factor was absent from nuclei, mitochondria, basolateral surface membranes, blunt microvilli, and the cytosol. Thus, we have defined the parietal cell as the site of IF synthesis. The observed distribution of intracellular IF suggested that an interrelationship existed between the stained membranous structures, and that IF secretion may depend upon membrane translocation. Further immunocytochemical and physiologic studies should increase our understanding of the cellular physiology of IF secretion as well as the parietal cell.