PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA.
PCNA structure and function: insights from structures of PCNA complexes and post-translationally modified PCNA.
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PCNA结构和功能：PCNA复合物结构和翻译后修饰的PCNA的见解。
DOI：
10.1007/978-94-007-4572-8_15
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发表时间：
2012
影响因子：
--
通讯作者：
Washington, M Todd
中科院分区：
文献类型：
--
作者：
Dieckman, Lynne M;Freudenthal, Bret D;Washington, M Todd
增殖细胞核抗原(增殖细胞核抗原)是真核细胞DNA的滑动夹，形成一个环状的同源三聚体，环绕双链DNA。这种蛋白质最为人所知的是其赋予复制DNA聚合酶高加工性的能力。然而，它的作用远远不止于此，因为它在DNA上形成了一个移动平台，招募了许多参与DNA复制、修复和重组的蛋白质到复制叉上。与增殖细胞核抗原结合蛋白结合的增殖细胞核抗原的X射线晶体结构提供了对增殖细胞核抗原如何识别其结合伙伴并将其招募到复制叉子上的洞察。最近，泛素修饰和相扑修饰的增殖细胞核抗原的X射线晶体结构提供了关于这些翻译后修饰如何改变某些结合伙伴的增殖细胞核抗原的特异性的见解。这篇文章集中在对增殖细胞核抗原复合体和翻译后修饰的增殖细胞核抗原的结构研究中获得的见解。
Proliferating cell nuclear antigen (PCNA), the eukaryotic DNA sliding clamp, forms a ring-shaped homo-trimer that encircles double-stranded DNA. This protein is best known for its ability to confer high processivity to replicative DNA polymerases. However, it does far more than this, because it forms a mobile platform on the DNA that recruits many of the proteins involved in DNA replication, repair, and recombination to replication forks. X-ray crystal structures of PCNA bound to PCNA-binding proteins have provided insights into how PCNA recognizes its binding partners and recruits them to replication forks. More recently, X-ray crystal structures of ubiquitin-modified and SUMO-modified PCNA have provided insights into how these post-translational modifications alter the specificity of PCNA for some of its binding partners. This article focuses on the insights gained from structural studies of PCNA complexes and post-translationally modified PCNA.