The barley UNICULM2 gene resides in a centromeric region and may be associated with signaling and stress responses
The barley UNICULM2 gene resides in a centromeric region and may be associated with signaling and stress responses
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DOI：
10.1007/s10142-012-0299-7
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发表时间：
2013-03-01
影响因子：
2.9
通讯作者：
Muehlbauer, Gary J.
中科院分区：
文献类型：
--
作者：
Okagaki, Ron J.;Cho, Seungho;Muehlbauer, Gary J.
营养体腋生分生组织（AXM）的活动导致分枝的产生。在大麦（Hordeum vulgare L.）中，营养体AXM在树冠中发育并产生被称为分蘖的变异枝。大麦低分蘖突变体uniculm 2中的突变阻断营养AXM发育并阻止分蘖发育。这项工作的目的是研究野生型和cul 2突变体植物中的基因表达，精细定位CUL 2基因，并研究大麦与水稻中CUL 2区域的同线性。RNA分析实验使用两个近等基因系对携带CUL 2突变等位基因或野生型CUL 2等位基因在不同的遗传背景中检测到28个独特的基因转录表现出类似的模式的差异积累在两种遗传背景，表明我们已经确定了关键基因的影响CUL 2基因。24个基因在uniculm 2突变体组织中具有更高的丰度，并且近一半的注释基因可能在应激反应或信号转导途径中起作用。遗传作图在1，088个F-2个体中鉴定了5个共分离标记。这些标记跨越染色体6 H上的着丝粒区域，并且与水稻2号染色体上的50-cM区域一致，这表明定位克隆CUL 2可能是困难的。总之，结果揭示了与CUL 2基因相关的应激反应和信号转导途径，通过可能困难的位置克隆方法分离CUL 2，以及CUL 2区域中大麦-水稻同线性的残余。
Vegetative axillary meristem (AXM) activity results in the production of branches. In barley (Hordeum vulgare L.), vegetative AXM develop in the crown and give rise to modified branches, referred to as tillers. Mutations in the barley low-tillering mutant uniculm2 block vegetative AXM development and prevent tiller development. The objectives of this work were to examine gene expression in wild-type and cul2 mutant plants, fine map the CUL2 gene, and to examine synteny in the CUL2 region in barley with rice. RNA profiling experiments using two near-isogenic line pairs carrying either the cul2 mutant allele or wild-type CUL2 allele in different genetic backgrounds detected 28 unique gene transcripts exhibiting similar patterns of differential accumulation in both genetic backgrounds, indicating that we have identified key genes impacted by the CUL2 gene. Twenty-four genes had higher abundance in uniculm2 mutant tissues, and nearly half of the annotated genes likely function in stress-response or signal transduction pathways. Genetic mapping identified five co-segregating markers in 1,088 F-2 individuals. These markers spanned the centromere region on chromosome 6H, and coincided with a 50-cM region on rice chromosome 2, indicating that it may be difficult to positionally clone CUL2. Taken together, the results revealed stress response and signal transduction pathways that are associated with the CUL2 gene, isolating CUL2 via positional cloning approaches that may be difficult, and the remnants of barley-rice synteny in the CUL2 region.