HUMAN-GENE ENCODING THE 78,000-DALTON GLUCOSE-REGULATED PROTEIN AND ITS PSEUDOGENE - STRUCTURE, CONSERVATION, AND REGULATION
HUMAN-GENE ENCODING THE 78,000-DALTON GLUCOSE-REGULATED PROTEIN AND ITS PSEUDOGENE - STRUCTURE, CONSERVATION, AND REGULATION
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DOI：
10.1089/dna.1988.7.275
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发表时间：
1988-05-01
期刊：
影响因子：
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通讯作者：
LEE, AS
中科院分区：
文献类型：
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作者：
TING, J;LEE, AS
描述了人功能性GRP78基因和加工假基因的分离和表征。我们提出了完整的一级结构的人GRP78基因，它跨越超过5 kb，由8个外显子。序列比较表明，GRP78基因在人类、大鼠和仓鼠的蛋白质编码区和3“非翻译区具有不寻常的同源性。此外，在GRP78的疏水区域内鉴定了与从人、果蝇、非洲爪蟾、酵母和大肠杆菌DNA分离的HSP 70高度保守的短结构域。无内含子假基因类似于加工基因。它的侧翼是一个短的直接重复序列，并嵌入在富含AT的基因组区域内。来自功能性人GRP78基因的高活性启动子包含TATA盒、五个CCAAT序列和两个转录因子Sp1的潜在结合位点。它由一个远端结构域，提高基础水平的表达和近端结构域必不可少的响应钙离子载体和温度敏感性突变，诱导GRP78基因。这两个结构域在大鼠和人GRP78启动子之间是高度保守的。
The isolation and characterization of a human functional GRP78 gene and a processed pseudogene are described. We present the complete primary structure of the human GRP78 gene, which spans over 5 kb and consists of eight exons. Sequence comparisons reveal that the GRP78 gene shares unusual homology among the human, rat, and hamster in the protein-coding and 3'' untranslated regions. In addition, short domains highly conserved with HSP70 isolated from human, Drosophila, Xenopus, yeast, and Escherichia coli DNA are identified within the hydrophobic regions of GRP78. The intronless pseudogene resembles that of a processed gene. It is flanked by a short direct repeat and is embedded within an AT-rich genomic region. The highly active promoter from the functional human GRP78 gene contains a TATA box, five CCAAT sequences, and two potential binding sites for the transcriptional factor Sp1. It consists of a distal domain that enhances basal level expression and a proximal domain essential for responses to calcium ionophore and for a temperature-sensitive mutation which induce the GRP78 gene. Both domains are highly conserved between the rat and the human GRP78 promoters.